Yin Jian, Wei Wanzhi, Liu Xiaoying, Kong Bo, Wu Ling, Gong Shuguo
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, People's Republic of China.
Anal Biochem. 2007 Jan 1;360(1):99-104. doi: 10.1016/j.ab.2006.10.007. Epub 2006 Oct 27.
A biosensor based on bovine serum albumin (BSA) for the detection of lead (Pb(2+)) ion was developed and characterized. BSA was immobilized onto a colloidal Au-modified piezoelectric quartz crystal (PQC) as a biosensor for the detection of Pb(2+) ion by piezoelectric quartz crystal impedance (PQCI). Calibration curves for the quantification of Pb(2+) ion showed excellent linearity throughout the concentration range from 1.0 x 10(-7) to 3.0 x 10(-9)mol/L. The interaction between the Pb(2+) ions and the sensor chip is influenced significantly by the pH of the reaction buffer, and the optimal pH for the experiment was 5.4. Under the optimal conditions, the detection limit of 1.0 x 10(-9)mol/L for Pb(2+) was obtained. Kinetic parameters of the Pb(2+)-BSA interactions were also determined by using this chip. The sensor chip could be regenerated for use by dipping in the ethylenediaminetetraacetic acid (EDTA) solution for approximately 2h, and the chip was used to detect Pb(2+) ion for eight times without obvious signal attenuation.
开发并表征了一种基于牛血清白蛋白（BSA）用于检测铅（Pb(2+)）离子的生物传感器。将BSA固定在胶体金修饰的压电石英晶体（PQC）上，作为通过压电石英晶体阻抗（PQCI）检测Pb(2+)离子的生物传感器。用于定量Pb(2+)离子的校准曲线在1.0×10(-7)至3.0×10(-9)mol/L的整个浓度范围内显示出优异的线性。反应缓冲液的pH值对Pb(2+)离子与传感器芯片之间的相互作用有显著影响，实验的最佳pH值为5.4。在最佳条件下，获得了Pb(2+)的检测限为1.0×10(-9)mol/L。还使用该芯片测定了Pb(2+)-BSA相互作用的动力学参数。通过将传感器芯片浸入乙二胺四乙酸（EDTA）溶液中约2小时可使其再生以供使用，并且该芯片用于检测Pb(2+)离子八次而没有明显的信号衰减。
