Vora Amit R, Bodell Sarah M, Loescher Alison R, Smith Keith G, Robinson Peter P, Boissonade Fiona M
Department of Oral and Maxillofacial Surgery, School of Clinical Dentistry, Claremont Crescent, Sheffield S10 2TA, UK.
Arch Oral Biol. 2007 Jan;52(1):74-82. doi: 10.1016/j.archoralbio.2006.08.015. Epub 2006 Nov 13.
To quantify the accumulation of inflammatory cells in traumatic neuromas of the human lingual nerve, and to establish any correlation with the patients' reported symptoms of dysaesthesia.
Using fluorescence immunohistochemistry, the extent of any chronic inflammatory infiltrate was quantified in human lingual neuroma specimens removed from 24 patients at the time of microsurgical nerve repair. A pan-leucocyte marker (CD45) and a specific macrophage marker (CD68) were used, and comparisons made between neuromas-in-continuity (NICs) and nerve-end neuromas (NENs) in patients with or without symptoms of dysaesthesia.
CD68 and CD45 labelling was significantly associated with areas of viable nerve tissue in neuromas and the CD68 labelling was significantly higher in NICs than NENs. CD68 labelling density tended to decrease with increasing time after the initial nerve injury, but this correlation was only significant for labelling associated with viable nerve tissue in NENs. No significant difference was found between the level of CD68 or CD45 labelling in patients with or without symptoms of dysaesthesia.
This study has demonstrated the presence of inflammatory cells within traumatic neuromas of the human lingual nerve. These cells were found to be closely associated with regions of viable nerve tissue, but there was no correlation with the patients' clinical symptoms.
量化人舌神经创伤性神经瘤中炎性细胞的积聚情况，并确定其与患者所报告的感觉异常症状之间的相关性。
采用荧光免疫组织化学方法，对24例患者在显微外科神经修复时切除的人舌神经瘤标本中慢性炎性浸润的程度进行量化。使用全白细胞标志物（CD45）和特异性巨噬细胞标志物（CD68），并对有或没有感觉异常症状的患者的连续性神经瘤（NICs）和神经末端神经瘤（NENs）进行比较。
CD68和CD45标记与神经瘤中存活神经组织区域显著相关，且NICs中的CD68标记显著高于NENs。CD68标记密度倾向于随着初始神经损伤后时间的增加而降低，但这种相关性仅在与NENs中存活神经组织相关的标记中显著。有或没有感觉异常症状的患者之间，CD68或CD45标记水平没有显著差异。
本研究证明了人舌神经创伤性神经瘤中存在炎性细胞。这些细胞与存活神经组织区域密切相关，但与患者的临床症状无关。
