Caldeira Eduardo José, Carvalho César Alexandre Fabrega, Padovani Carlos Roberto, Camilli José Angelo, Garcia Progresso José, Cagnon Valéria Helena Alves
Department of Anatomy, Institute of Biology, State University of Campinas, UNICAMP, Campinas, São Paulo, Brazil.
Arch Oral Biol. 2007 Jan;52(1):83-9. doi: 10.1016/j.archoralbio.2006.07.008. Epub 2006 Nov 13.
Smoking is considered to be the most albeit preventable cause of diseases and premature deaths in the history of mankind. The local action of tobacco on the oral mucosa can cause precancerous and cancerous lesions. However, there is not enough evidence to establish all the systemic effects caused by nicotine on the organism. Thus, the aim of the present study was to characterize the cellular changes of the cheek mucosa of rats submitted to long-term systemic nicotine treatment. Twenty male rats were divided into two experimental groups: a nicotine group and a control group, each consisting of 10 animals. The nicotine group was injected daily with 0.250 mg of nicotine per 100 g of body weight. All animals received a solid diet and water ad libitum. After 90 days of treatment, all animals were weighed and sacrificed. Samples of cheek mucosa were collected for light and transmission electron microscopy. The results revealed oral epithelium containing atypical cells that were characterized by atrophy, cell membrane disorganization and tissue damage. It was concluded that systemic administration of nicotine damaged the cellular integrity of the oral mucosa, impairing tissue function and predisposing the tissue to the action of different pathogenic agents and also to that of other carcinogenic substances present in tobacco.
吸烟被认为是人类历史上尽管可预防但却是导致疾病和过早死亡的最主要原因。烟草对口腔黏膜的局部作用可导致癌前病变和癌变。然而，尚无足够证据证实尼古丁对机体产生的所有系统性影响。因此，本研究的目的是描述长期接受系统性尼古丁治疗的大鼠颊黏膜的细胞变化。将20只雄性大鼠分为两个实验组：尼古丁组和对照组，每组10只动物。尼古丁组每天每100克体重注射0.250毫克尼古丁。所有动物均自由摄取固体食物和水。治疗90天后，称量所有动物体重并将其处死。采集颊黏膜样本用于光镜和透射电镜检查。结果显示口腔上皮含有非典型细胞，其特征为萎缩、细胞膜紊乱和组织损伤。得出的结论是，系统性给予尼古丁会损害口腔黏膜的细胞完整性，损害组织功能，并使该组织易受不同病原体以及烟草中存在的其他致癌物质的影响。
