Kicman Andrew T, Parkin Mark C, Iles Ray K
Department of Forensic Science & Drug Monitoring (Drug Control Centre), Kings College London, 150 Stamford Street, London SE1 9NH, UK.
Mol Cell Endocrinol. 2007 Jan 2;260-262:212-27. doi: 10.1016/j.mce.2006.02.022. Epub 2006 Nov 13.
This review introduces fundamental aspects of mass spectrometry (MS) based proteomics and illustrates how MS is an effective tool for the analysis of glycoprotein hormones. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) MS are complementary approaches that have been applied for the analysis of gonadotropins, e.g. to characterize differences in the oligosaccharide distribution of commercial human chorionic gonadotropin preparations, for isolated nicked beta-subunit, and identification of a metabolite of placental transforming growth factor in pharmaceutical hCG preparations. Immunoaffinity trapping and concentration of digested sample extract prior to MS analysis confers analytical sensitivity akin to immunoassay. A desirable objective would be to develop for clinical purposes a rapid procedure for MS detection and characterization of gonadotropins. Refinement of on-target immobilization and digestion for subsequent ionization by MALDI may eventually help to provide this capability. The advent of hybrid mass spectrometers will further advance the characterization of these complex molecules.
本综述介绍了基于质谱（MS）的蛋白质组学的基本方面，并说明了质谱如何成为分析糖蛋白激素的有效工具。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱和电喷雾电离（ESI）质谱是互补的方法，已应用于促性腺激素的分析，例如用于表征市售人绒毛膜促性腺激素制剂中寡糖分布的差异、用于分离的切口β亚基，以及鉴定药物性人绒毛膜促性腺激素制剂中胎盘转化生长因子的一种代谢物。在质谱分析之前，通过免疫亲和捕获和浓缩消化后的样品提取物可赋予类似于免疫测定的分析灵敏度。一个理想的目标是为临床目的开发一种用于质谱检测和表征促性腺激素的快速程序。改进用于随后通过MALDI进行电离的靶上固定和消化最终可能有助于提供这种能力。混合质谱仪的出现将进一步推动对这些复杂分子的表征。
