Liesker J J W, Ten Hacken N H T, Rutgers S R, Zeinstra-Smith M, Postma D S, Timens W
Department of Pulmonology, University Medical Center Groningen, University of Groningen, the Netherlands.
Respir Med. 2007 May;101(5):882-7. doi: 10.1016/j.rmed.2006.10.001. Epub 2006 Nov 13.
Most patients with asthma and many patients with COPD show bronchial hyperresponsiveness to adenosine (BHR(AMP)). BHR(AMP) may be caused by release of mast cell histamine, which induces smooth muscle contraction.
To evaluate whether mast cell numbers in airway smooth muscle are increased in patients with asthma and COPD compared to their age-matched controls, and whether mast cell numbers are correlated with BHR(AMP).
Twenty-two non-smoking subjects with asthma (age 31yr, FEV(1): 89% pred, PC(20)AMP: 2.7mg/ml), 18 ex-smoking subjects with COPD (age 62yr, FEV(1): 58% pred, PC(20)AMP: 52.4mg/ml).
Snap-frozen bronchial biopsies were immunostained with anti-mast cell tryptase and anti-desmin antibodies. Mast cell number was expressed as the number of tryptase positive cells per area of smooth muscle.
There were no significant differences in mast cell number between patients with asthma, COPD, and their respective age-matched healthy controls. Furthermore, there was no significant correlation between mast cell number and FEV(1) or PC(20)AMP in any of the groups. Surprisingly, the mast cell number was negatively correlated with reversibility to salbutamol in COPD patients (rho -0.47, P<0.05).
Mast cell numbers in central airway smooth muscle apparently do not contribute importantly to bronchial hyperresponsiveness to adenosine.
大多数哮喘患者以及许多慢性阻塞性肺疾病（COPD）患者表现出对腺苷的支气管高反应性（BHR(AMP)）。BHR(AMP)可能由肥大细胞组胺释放引起，组胺可诱导平滑肌收缩。
评估与年龄匹配的对照组相比，哮喘和COPD患者气道平滑肌中的肥大细胞数量是否增加，以及肥大细胞数量是否与BHR(AMP)相关。
22名非吸烟哮喘患者（年龄31岁，第一秒用力呼气容积（FEV(1)）：占预计值的89%，腺苷激发试验浓度为20%时的累积激发剂量（PC(20)AMP）：2.7mg/ml），18名已戒烟的COPD患者（年龄62岁，FEV(1)：占预计值的58%，PC(20)AMP：52.4mg/ml）。
将速冻的支气管活检组织用抗肥大细胞类胰蛋白酶和抗结蛋白抗体进行免疫染色。肥大细胞数量以每平滑肌面积中类胰蛋白酶阳性细胞的数量表示。
哮喘患者、COPD患者与其各自年龄匹配的健康对照组之间的肥大细胞数量无显著差异。此外，在任何一组中，肥大细胞数量与FEV(1)或PC(20)AMP之间均无显著相关性。令人惊讶的是，在COPD患者中，肥大细胞数量与沙丁胺醇的可逆性呈负相关（相关系数ρ为 -0.47，P<0.05）。
中央气道平滑肌中的肥大细胞数量显然对支气管对腺苷的高反应性没有重要贡献。
