Kalahasthi Ravi Babu, Rao Rajmohan Hirehal Raghavendra, Murthy Rajan Bagalur Krishna, Kumar M Karuna
Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bangalore Medical College Campus, Bangalore, India.
Chem Biol Interact. 2006 Dec 15;164(3):192-9. doi: 10.1016/j.cbi.2006.09.012. Epub 2006 Oct 6.
The present study was carried out to determine the effect of chromium(VI) on the status of plasma lipid peroxidation and erythrocyte antioxidant enzymes in workers exposed to chromium during chromium plating process.
Fifty subjects working in chromium plating process formed the study group. An equal number of age-sex matched subjects working in administrative units formed the control group. The control subjects were residing in the same city but away from the work place of study group subjects. Urinary chromium levels were determined by using a graphite furnace atomic absorption spectrophotometer. The plasma lipid peroxidation and erythrocyte antioxidant enzymes were determined by using spectrophotmetric methods.
A significant increase of plasma lipid peroxidation and a significant decrease of superoxide dismutase and glutathione peroxidase levels were noted in the study group as compared with the controls. The level of plasma lipid peroxidation was positively and erythrocyte antioxidant enzymes were negatively and significantly correlated with chromium levels in urine. Multiple regression analysis was assessed the oxidative stress associated with chromium and life style confounding factors such as BMI, coffee, tea, alcohol and smoking. The multiple regression analysis showed that the urine chromium levels >10 micro g/g of creatinine, smoking, consumption of green vegetables and BMI variables were significantly associated with the levels of oxidative stress.
The results show that the increased plasma lipid peroxidation and decreased antioxidant enzymes (superoxide dismutase and glutathione peroxidase) observed in chromium-exposed workers could be used as biomarkers of oxidative stress.
本研究旨在确定六价铬对镀铬过程中接触铬的工人血浆脂质过氧化状态和红细胞抗氧化酶的影响。
五十名从事镀铬工作的受试者组成研究组。同等数量年龄和性别匹配的在行政单位工作的受试者组成对照组。对照组受试者居住在同一城市，但远离研究组受试者的工作场所。采用石墨炉原子吸收分光光度计测定尿铬水平。采用分光光度法测定血浆脂质过氧化和红细胞抗氧化酶。
与对照组相比，研究组血浆脂质过氧化显著增加，超氧化物歧化酶和谷胱甘肽过氧化物酶水平显著降低。血浆脂质过氧化水平与尿铬水平呈正相关，红细胞抗氧化酶与尿铬水平呈负相关且具有显著相关性。多元回归分析评估了与铬以及体重指数、咖啡、茶、酒精和吸烟等生活方式混杂因素相关的氧化应激。多元回归分析表明，尿铬水平>10微克/克肌酐、吸烟、绿色蔬菜摄入量和体重指数变量与氧化应激水平显著相关。
结果表明，在接触铬的工人中观察到的血浆脂质过氧化增加和抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）减少可作为氧化应激的生物标志物。
