Kalahasthi Ravi Babu, Hirehal Raghavendra Rao Rajmohan, Bagalur Krishna Murthy Rajan
Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bangalore Medical College Campus, Bangalore, India.
Biomarkers. 2006 May-Jun;11(3):241-9. doi: 10.1080/13547500600633897.
The objectives were to investigate the plasma lipid peroxidation and erythrocyte antioxidants status in workers exposed to nickel. The study groups comprised 69 nickel plating workers and 50 office workers residing in the same city, but away from the place of work of the study group subjects, considered as control group. Urinary nickel concentration was determined by graphite furnace atomic absorption spectrophotometry. The plasma lipid peroxidation and erythrocyte antioxidants were measured by spectrophotmetric methods. The plasma lipid peroxidation level was significantly increased in nickel-platers and their helpers as compared with controls. Erythrocyte antioxidants were significantly decreased in the nickel-platers compared with the controls. The level of plasma lipid peroxidation was positively and erythrocyte antioxidants were negatively and significantly correlated with the urine nickel levels. Multiple regression analysis assessed the oxidative stress associated with nickel and other potential confounding factors such as body mass index, the consumption of green vegetables, coffee, tea, smoking and alcohol consumption. Analysis showed that the lifestyle confounding factors: the consumption of green vegetables, smoking and alcohol, were not significantly associated with oxidative stress. The exposure to nickel, body mass index and coffee consumption were significantly associated with oxidative stress. The results show that the increased plasma lipid peroxidation and decreased erythrocyte antioxidants levels observed in nickel-exposed workers could be used as biomarkers of oxidative stress.
目的是调查接触镍的工人的血浆脂质过氧化和红细胞抗氧化剂状态。研究组包括69名镀镍工人和50名居住在同一城市但远离研究组受试者工作地点的办公室职员，后者被视为对照组。尿镍浓度通过石墨炉原子吸收分光光度法测定。血浆脂质过氧化和红细胞抗氧化剂通过分光光度法测量。与对照组相比，镀镍工人及其助手的血浆脂质过氧化水平显著升高。与对照组相比，镀镍工人的红细胞抗氧化剂显著降低。血浆脂质过氧化水平与尿镍水平呈正相关，而红细胞抗氧化剂与尿镍水平呈负相关且具有显著相关性。多元回归分析评估了与镍以及其他潜在混杂因素（如体重指数、绿色蔬菜摄入量、咖啡、茶、吸烟和饮酒量）相关的氧化应激。分析表明，生活方式混杂因素：绿色蔬菜摄入量、吸烟和饮酒，与氧化应激无显著关联。镍暴露、体重指数和咖啡摄入量与氧化应激显著相关。结果表明，在接触镍的工人中观察到的血浆脂质过氧化增加和红细胞抗氧化剂水平降低可作为氧化应激的生物标志物。
