Conceição Katia, Konno Katsuhiro, de Melo Robson Lopes, Antoniazzi Marta M, Jared Carlos, Sciani Juliana M, Conceição Isaltino M, Prezoto Benedito C, de Camargo Antônio Carlos Martins, Pimenta Daniel C
Laboratório Especial de Toxinologia Aplicada, Center for Applied Toxinology (CAT/CEPID), Instituto Butantan, Avenida Vital Brasil 1500, São Paulo, SP 05503-900, Brazil.
Peptides. 2007 Mar;28(3):515-23. doi: 10.1016/j.peptides.2006.10.002. Epub 2006 Nov 13.
Bradykinin potentiating peptides (BPPs) from Bothrops jararaca venom were first described in the middle of 1960s and were the first natural inhibitors of the angiotensin-converting enzyme (ACE). BPPs present a classical motif and can be recognized by their typical pyroglutamyl (Pyr)/proline rich sequences presenting, invariably, a proline residue at the C-terminus. In the present study, we describe the isolation and biological characterization of a novel BPP isolated from the skin secretion of the Brazilian tree-frog Phyllomedusa hypochondrialis. This new BPP, named Phypo Xa presents the sequence Pyr-Phe-Arg-Pro-Ser-Tyr-Gln-Ile-Pro-Pro and is able to potentiate bradykinin activities in vivo and in vitro, as well as efficiently and competitively inhibit ACE. This is the first canonical BPP (i.e. Pyr-Aaa(n)-Gln-Ile-Pro-Pro) to be found not only in the frog skin but also in any other natural source other than the snake venoms.
来自巴西矛头蝮蛇毒液的缓激肽增强肽（BPPs）于20世纪60年代中期首次被描述，是最早的血管紧张素转换酶（ACE）天然抑制剂。BPPs具有一个经典基序，可通过其典型的焦谷氨酰（Pyr）/富含脯氨酸的序列识别，在C末端总是存在一个脯氨酸残基。在本研究中，我们描述了从巴西树蛙（Phyllomedusa hypochondrialis）皮肤分泌物中分离出的一种新型BPP的分离及生物学特性。这种新的BPP名为Phypo Xa，其序列为Pyr-Phe-Arg-Pro-Ser-Tyr-Gln-Ile-Pro-Pro，能够在体内和体外增强缓激肽活性，以及有效且竞争性地抑制ACE。这是首次在青蛙皮肤中发现，也是在蛇毒以外的任何其他天然来源中发现的首个典型BPP（即Pyr-Aaa(n)-Gln-Ile-Pro-Pro）。
