Fu Shu-Lan, Tang Zong-Xiang, Zhang Huai-Qiong, Yang Zu-Jun, Ren Zheng-Long
State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya'an 625014, China.
Yi Chuan. 2006 Nov;28(11):1396-400. doi: 10.1360/yc-006-1396.
The advanced progeny lines (BC1F5) from the monosomic addition lines between common wheat cultivar Mianyang 11, which is highly susceptible to powdery mildew, and an inbred rye line R12 were analyzed for selection of wheat-rye translocations. Based on a rye-specific repetitive sequence of pSc20H, which spread over all chromosomes of rye but did not existed in wheat, a set of PCR primer was designed and used to identify the rye chromosome segments in wheat. From 300 of the BC1F5 progeny lines 70 were found to contain chromosome composition of rye. An advanced line, 96II691-830-98, originated from 6R monosomic addition line was observed to be immune to powdery mildew, different from its wheat parent Mianyang 11. A small segment of rye chromosome at telomere in a pair of wheat chromosome in the line was found by means of GISH. The results indicated that a small segment of rye chromosome 6R carrying the gene(s) for resistance to powdery mildew has been transferred into common wheat. In the progeny of monosomic addition lines a high frequency of wheat-alien species translocation with various segments of chromosomes could be found by application of both PCR and GISH technique.
以高感白粉病的普通小麦品种绵阳11与黑麦自交系R12的单体附加系的高世代后代株系（BC1F5）为材料，进行小麦-黑麦易位系的筛选。根据黑麦特异的重复序列pSc20H（其分布于黑麦的所有染色体上，而小麦中不存在）设计了一套PCR引物，用于鉴定小麦中的黑麦染色体片段。在300个BC1F5后代株系中，发现70个含有黑麦染色体组成。来源于6R单体附加系的一个高世代株系96II691-830-98表现出对白粉病免疫，与其小麦亲本绵阳11不同。通过基因组原位杂交（GISH）发现该株系一对小麦染色体的端粒处有一小段黑麦染色体。结果表明，携带白粉病抗性基因的一小段黑麦6R染色体已导入普通小麦。在单体附加系后代中，应用PCR和GISH技术可发现较高频率的带有不同染色体片段的小麦-外源物种易位。
