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多胺和双（胍基腙）甲基乙二醛对肝细胞核结构及脱氧核糖核酸模板活性的影响。

Effects of polyamines and methylglyoxal bis(guanylhydrazone) on hepatic nuclear structure and deoxyribonucleic acid template activity.

作者信息

Brown K B, Nelson N F, Brown D G

出版信息

Biochem J. 1975 Dec;151(3):505-12. doi: 10.1042/bj1510505.

DOI:10.1042/bj1510505
PMID:1218090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172397/
Abstract
  1. The interaction of polyamines and methylglyoxal bis(guanythydrazone) (1, 1'-[(methylethanediylidene)-dinitrilo]diguanidine) with isolated rat liver nuclei was investigated by electron microscopy. 2. At 4mM, putrescine was without effect; however, spermidine, spermine or methylglyoxal bis(guanythydrazone) resulted in dispersed chromatin and alterations in nucleolar structure. In addition, spermidine or methylglyoxal bis(guanylhydrazone) caused marked aggregation of interchromatin granules. 3. The DNA template property of calf thymus DNA was examined by using DNA polymerases from Escherichia coli, Micrococcus lysodeikticus and calf thymus in the presence of 0-5 mM-amine. 4. In the presence of DNA polymerase, spermine or methylglyoxal bis(guanylhydrazone) inhibited activity, whereas putrescine or spermidine had much less effect or in some cases stimulated [3H]dTMP incorporation. 5. Template activity which was inhibited by spermine or methylglyoxal bis(guanylhydrazone) could be partially restored by additional DNA or enzyme. 6. When mixed with calf thymus DNA, calf thymus histone inhibited template activity as measured with E. coli DNA polymerase. The template activity of such a 'histone-nucleate' could not be restored by putrescine, spermidine, spermine or methylglyoxal bis(guanylhydrazone). 7. DNA template activity of isolated rat liver nuclei was tested by using E. coli DNA polymerase. None of the amines was able to increase the template activity of the nuclear DNA in vitro.
摘要
  1. 通过电子显微镜研究了多胺与甲基乙二醛双（胍基腙）（1,1'-[(甲基亚乙基)-二亚氨基]二胍）与分离的大鼠肝细胞核的相互作用。2. 在4mM时，腐胺无作用；然而，亚精胺、精胺或甲基乙二醛双（胍基腙）导致染色质分散和核仁结构改变。此外，亚精胺或甲基乙二醛双（胍基腙）引起染色质间颗粒明显聚集。3. 在0 - 5mM胺存在的情况下，使用来自大肠杆菌、溶壁微球菌和小牛胸腺的DNA聚合酶检测小牛胸腺DNA的DNA模板特性。4. 在DNA聚合酶存在的情况下，精胺或甲基乙二醛双（胍基腙）抑制活性，而腐胺或亚精胺的作用小得多，在某些情况下还刺激[3H]dTMP掺入。5. 被精胺或甲基乙二醛双（胍基腙）抑制的模板活性可通过额外的DNA或酶部分恢复。6. 当与小牛胸腺DNA混合时，小牛胸腺组蛋白抑制用大肠杆菌DNA聚合酶测量的模板活性。这种“组蛋白核”的模板活性不能被腐胺、亚精胺、精胺或甲基乙二醛双（胍基腙）恢复。7. 使用大肠杆菌DNA聚合酶测试分离的大鼠肝细胞核的DNA模板活性。在体外，没有一种胺能够增加核DNA的模板活性。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdc5/1172397/e9a7fc780bdc/biochemj00547-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdc5/1172397/e9a7fc780bdc/biochemj00547-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdc5/1172397/e9a7fc780bdc/biochemj00547-0059-a.jpg

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本文引用的文献

1
COMPLEX FORMATION WITH DNA AND INHIBITION OF NUCLEIC ACID SYNTHESIS BY METHYLGLYOXAL BIS(GUANYLHYDRAZONE).甲基乙二醛双（脒腙）与DNA的复合物形成及对核酸合成的抑制作用
Biochim Biophys Acta. 1965 May 11;103:174-6. doi: 10.1016/0005-2787(65)90550-2.
2
CURRENT STUDIES WITH METHYLGLYOXAL-BIS(GUANYLHYDRAZONE).甲基乙二醛双（脒腙）的当前研究
Cancer Res. 1963 Sep;23:1375-89.
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A microchemical determination of desoxyribonucleic acid.脱氧核糖核酸的微量化学测定
Nucleic Acids Res. 1977 Mar;4(3):685-95. doi: 10.1093/nar/4.3.685.
J Biol Chem. 1952 Sep;198(1):297-303.
4
Effect of inhibition of spermidine formation on protein and nucleic acid synthesis during lymphocyte activation.抑制亚精胺形成对淋巴细胞激活过程中蛋白质和核酸合成的影响。
FEBS Lett. 1973 Feb 1;29(3):301-304. doi: 10.1016/0014-5793(73)80044-4.
5
Interaction of spermine and DNA.精胺与DNA的相互作用。
Biopolymers. 1967 Feb;5(2):227-33. doi: 10.1002/bip.1967.360050209.
6
Nuclei from rat liver: isolation method that combines purity with high yield.大鼠肝脏细胞核：一种将纯度与高产率相结合的分离方法。
Science. 1966 Dec 30;154(3757):1662-5. doi: 10.1126/science.154.3757.1662.
7
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Biochem J. 1970 Mar;117(1):17-31. doi: 10.1042/bj1170017.
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