Villareal Arturo L, Farley William, Pflugfelder Stephen C
Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA.
Eye Contact Lens. 2006 Dec;32(6):272-6. doi: 10.1097/01.icl.0000224360.10319.b1.
To investigate the effects of topical epinastine and olopatadine on tear volume by using a mouse model.
Eighty-five C57BL6 mice (170 eyes) were treated twice daily with topical ophthalmic epinastine 0.05%, olopatadine 0.1%, or atropine 1% or served as untreated controls. A thread-wetting assay was used to measure tear volume at baseline and 15, 45, 90, 120, and 240 minutes after the last instillation of the drug on days 2 and 4 of treatment.
After 2 days of treatment, epinastine-treated mice showed greater mean tear volumes than olopatadine-treated mice did at 15, 45, 90, and 240 minutes, with statistical significance at 15 and 45 minutes (P<0.001). Olopatadine significantly reduced tear volume versus untreated controls at 15 and 45 minutes (P<0.001). After 4 days, tear volumes with epinastine treatment exceeded those with olopatadine treatment at all time points, with statistical significance at 45 minutes (P<0.05). Atropine rendered tears undetectable at 15, 45, and 90 minutes; tear volume returned to baseline levels at 240 minutes.
Topical epinastine did not inhibit tear secretion, whereas olopatadine caused a significant decrease in tear volume. Because of its neutral impact on the lacrimal functional unit, epinastine may be an especially good choice for the treatment of allergic conjunctivitis in patients with dry eye disease or in those who are at risk for developing dry eye.
通过使用小鼠模型研究局部应用依匹斯汀和奥洛他定对泪液量的影响。
85只C57BL6小鼠（170只眼）每天接受两次局部应用0.05%依匹斯汀滴眼液、0.1%奥洛他定滴眼液或1%阿托品滴眼液治疗，或作为未治疗对照组。在治疗第2天和第4天，在末次滴药后基线以及15、45、90、120和240分钟时，采用线湿试验测量泪液量。
治疗2天后，依匹斯汀治疗组小鼠在15、45、90和240分钟时的平均泪液量高于奥洛他定治疗组小鼠，在15和45分钟时具有统计学意义（P<0.001）。与未治疗对照组相比，奥洛他定在15和45分钟时显著降低了泪液量（P<0.001）。治疗4天后，依匹斯汀治疗组在所有时间点的泪液量均超过奥洛他定治疗组，在45分钟时具有统计学意义（P<0.05）。阿托品使15、45和90分钟时的泪液无法检测到；240分钟时泪液量恢复到基线水平。
局部应用依匹斯汀不抑制泪液分泌，而奥洛他定可导致泪液量显著减少。由于依匹斯汀对泪腺功能单位的中性影响，它可能是治疗干眼症患者或有患干眼症风险患者过敏性结膜炎的特别好的选择。
