Wang Z Z, Stensaas L J, Dinger B, Fidone S J
Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108.
J Auton Nerv Syst. 1991 Mar;32(3):259-64. doi: 10.1016/0165-1838(91)90120-r.
Catecholamines are thought to play an important role in sensory transduction in the arterial chemoreceptors of the mammalian carotid body, and classical cytochemical techniques have demonstrated their presence in the type I (glomus) cells of this organ. However, it remains controversial whether dopamine (DA) and norepinephrine (NE) occur in the same or in different subtypes of glomus cells. In the present study, we have addressed this issue using immunocytochemistry to compare the localization of tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (D beta H) in the cat carotid body. Both pre- and post-embedding double-labelling immunohistochemical techniques were employed. TH and D beta H were found to co-exist in over 90% of the glomus cells, and they were co-localized at equivalent levels in almost 80% of the cells; less than 5% contained only TH. The results suggest that DA and NE are synthesized and stored in a common cell population in the cat carotid body.
儿茶酚胺被认为在哺乳动物颈动脉体的动脉化学感受器的感觉转导中起重要作用，经典的细胞化学技术已证明它们存在于该器官的I型（球）细胞中。然而，多巴胺（DA）和去甲肾上腺素（NE）是否存在于同一亚型或不同亚型的球细胞中仍存在争议。在本研究中，我们使用免疫细胞化学方法来比较猫颈动脉体中酪氨酸羟化酶（TH）和多巴胺β-羟化酶（DβH）的定位，以解决这个问题。我们采用了包埋前和包埋后双重标记免疫组织化学技术。发现超过90%的球细胞中TH和DβH共存，并且在近80%的细胞中它们以相同水平共定位；不到5%的细胞仅含有TH。结果表明，DA和NE在猫颈动脉体的共同细胞群体中合成和储存。
