Kato Kouki, Fushuku Seigo, Yamamoto Yoshio
Center for Laboratory Animal Science, National Defense Medical College, Tokorozawa, Japan.
Center for Laboratory Animal Science, National Defense Medical College, Tokorozawa, Japan.
Auton Neurosci. 2017 Jul;205:50-56. doi: 10.1016/j.autneu.2017.04.005. Epub 2017 Apr 28.
The purpose of this study was to investigate immunoreactivity for dopamine β-hydroxylase (DBH) and tyrosine hydroxylase (TH) in carotid body (CB) glomus cells in spontaneously hypertensive rats (SHR/Izm) at 4 (prehypertensive stage), 8 (early stage of developmental hypertension), 12 (later stage of developmental hypertension), and 16weeks of age (established hypertensive stage). Age-matched Wistar Kyoto rats (WKY/Izm) were used as controls. Staining properties for TH were similar between both strains at each age. Regarding DBH immunostaining, although some glomus cells showed intense DBH immunoreactivity at 4weeks of age, these cells were rarely observed at 8, 12, and 16weeks of age in WKY/Izm. In SHR/Izm, intense DBH immunoreactivity was observed in some glomus cells at 4weeks of age, these cells were also observed at 8 and 12weeks of age, and their number increased at 16weeks of age. An image analysis showed that the percentage of DBH-immunopositive glomus cells in WKY/Izm was approximately 30% at 4weeks of age and significantly decreased to approximately 10% at 8, 12, and 16weeks of age (p<0.05). This percentage in SHR/Izm was approximately 40% at each age. The gray scale intensity for DBH immunoreactivity in DBH-immunopositive glomus cells was similar in both strains at 4weeks of age, but became significantly lower in WKY/Izm and higher in SHR/Izm with increase in age (p<0.05). These results suggest that noradrenaline in glomus cells plays an important role in the regulation of neurotransmission between CB and afferent nerves during developmental hypertension.
本研究的目的是调查4周龄（高血压前期）、8周龄（发育性高血压早期）、12周龄（发育性高血压后期）和16周龄（确立高血压期）的自发性高血压大鼠（SHR/Izm）颈动脉体（CB）球细胞中多巴胺β-羟化酶（DBH）和酪氨酸羟化酶（TH）的免疫反应性。将年龄匹配的Wistar Kyoto大鼠（WKY/Izm）作为对照。在每个年龄阶段，两种品系的TH染色特性相似。关于DBH免疫染色，虽然在4周龄时一些球细胞显示出强烈的DBH免疫反应性，但在WKY/Izm的8周龄、12周龄和16周龄时很少观察到这些细胞。在SHR/Izm中，4周龄时在一些球细胞中观察到强烈的DBH免疫反应性，在8周龄和12周龄时也观察到这些细胞，并且其数量在16周龄时增加。图像分析显示，WKY/Izm中DBH免疫阳性球细胞的百分比在4周龄时约为30%，在8周龄、12周龄和16周龄时显著下降至约10%（p<0.05）。SHR/Izm中每个年龄的该百分比约为40%。在4周龄时，DBH免疫阳性球细胞中DBH免疫反应性的灰度强度在两种品系中相似，但随着年龄的增加，WKY/Izm中显著降低，而SHR/Izm中则升高（p<0.05）。这些结果表明，在发育性高血压期间，球细胞中的去甲肾上腺素在CB与传入神经之间的神经传递调节中起重要作用。
