利用基因敲除技术解决药理学问题。
Use of knockout technology to resolve pharmacological problems.
作者信息
Docherty J R
机构信息
Department of Physiology, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland.
出版信息
Br J Pharmacol. 2007 Jan;150(1):1-2. doi: 10.1038/sj.bjp.0706941. Epub 2006 Nov 13.
Abstract
Knock-out (KO) mouse technology has given pharmacologists a powerful tool to study function in the absence of selective antagonists or inhibitors. Such KO technology can confirm predicted function, serendipitously reveal unrecognized function, or help define the mode of action of a drug. In this issue, Liles et al. demonstrate, employing mice unable to synthesize noradrenaline due to the KO of the dopamine-beta-hydroxylase gene, that the sympathomimetic actions of ephedrine are directly, rather than indirectly, mediated. This may end 50 years of debate about the actions of ephedrine.
摘要
基因敲除（KO）小鼠技术为药理学家提供了一个强大的工具，用于在没有选择性拮抗剂或抑制剂的情况下研究功能。这种基因敲除技术可以证实预测的功能，意外地揭示未被认识到的功能，或者有助于确定药物的作用方式。在本期中，莱尔斯等人利用由于多巴胺-β-羟化酶基因敲除而无法合成去甲肾上腺素的小鼠，证明麻黄碱的拟交感神经作用是直接介导的，而非间接介导。这可能会结束长达50年关于麻黄碱作用的争论。
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Use of knockout technology to resolve pharmacological problems.利用基因敲除技术解决药理学问题。
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本文引用的文献
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Br J Pharmacol. 2007 Jan;150(1):29-36. doi: 10.1038/sj.bjp.0706942. Epub 2006 Nov 13.
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Pressor responses to ephedrine are mediated by a direct mechanism in the rat.在大鼠中，麻黄碱的升压反应是由直接机制介导的。
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All three alpha2-adrenoceptor types serve as autoreceptors in postganglionic sympathetic neurons.所有三种α2 -肾上腺素能受体类型在节后交感神经元中均作为自身受体发挥作用。
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The sympathomimetic actions of l-ephedrine and d-pseudoephedrine: direct receptor activation or norepinephrine release?左旋麻黄碱和右旋伪麻黄碱的拟交感神经作用：直接受体激活还是去甲肾上腺素释放？
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The effect of denervation on the action of sympathomimetic amines on the nictitating membrane.去神经对拟交感胺类药物作用于瞬膜的影响。
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In vitro characterization of ephedrine-related stereoisomers at biogenic amine transporters and the receptorome reveals selective actions as norepinephrine transporter substrates.麻黄碱相关立体异构体在生物胺转运体和受体组的体外特性研究揭示了其作为去甲肾上腺素转运体底物的选择性作用。
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