超氧化物歧化酶的酶法和非酶法测定
Enzymatic and non-enzymatic assay of superoxide dismutase.
作者信息
Fried R
出版信息
Biochimie. 1975;57(5):657-60. doi: 10.1016/s0300-9084(75)80147-7.
PMID:171001
Abstract
Superoxide dismutase from breef brain and rat liver was assayed in an enzymatic system, using xanthine oxidase, and a non-enzymatic system, based on aerobic reduction of nitro-blue tetrazolium in presence of phenazine methosulphate. The non-enzymatic assay is rapid and simple and permits simulatneous analysis of many samples. Similar results are found by the two methods of assay of superoxide dismutase.
摘要
在酶促体系中，利用黄嘌呤氧化酶对来自牛脑和大鼠肝脏的超氧化物歧化酶进行了测定；在非酶促体系中，基于在硫酸吩嗪存在下硝基蓝四氮唑的需氧还原对其进行了测定。非酶促测定方法快速、简便，可同时分析多个样品。两种超氧化物歧化酶测定方法得到了相似的结果。
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