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Inactivation of DNA polymerase by adenosine 2',3'-riboepoxide 5'-triphosphate allows estimation of the primers affinity.

作者信息

Podust V N, Korobeinicheva T O, Nevinsky G A, Levina A S, Lavrik O I

机构信息

Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.

出版信息

Mol Biol Rep. 1990 Nov;14(4):247-9. doi: 10.1007/BF00429892.

DOI:10.1007/BF00429892
PMID:1710018
Abstract

Template-primer dependent inactivation of human DNA polymerase alpha and Klenow fragment of E. coli DNA polymerase I by adenosine 2',3'-riboepoxide 5'-triphosphate was used for quantitative analysis of the Kd values for oligonucleotide primers of different length. The Kd values are smaller by a factor of 2.5 than the Km values for the same primers determined in the reaction of DNA polymerization in the case of DNA polymerase alpha. The Kd and Km values are nearly the same for Klenow fragment. Such approach to the determination of Km/Kd ratio can likely be used for detailed quantitative analysis of DNA polymerases.

摘要

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本文引用的文献

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Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase.甲磺酸酯作为乙酰胆碱酯酶的不可逆抑制剂
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Protein-nucleic acid interaction in reactions catalyzed with DNA polymerases.DNA聚合酶催化反应中的蛋白质-核酸相互作用。
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The efficiency of interaction of deoxyribonucleoside-5'-mono-, di- and triphosphates with the active centre of E. coli DNA polymerase I Klenow fragment.脱氧核糖核苷-5'-单磷酸、二磷酸和三磷酸与大肠杆菌DNA聚合酶I Klenow片段活性中心相互作用的效率。
FEBS Lett. 1989 Dec 18;259(1):83-5. doi: 10.1016/0014-5793(89)81500-5.
8
Inactivation of DNA polymerase I (Klenow fragment) by adenosine 2',3'-epoxide 5'-triphosphate: evidence for the formation of a tight-binding inhibitor.2',3'-环氧腺苷5'-三磷酸对DNA聚合酶I（克列诺片段）的失活作用：形成紧密结合抑制剂的证据
Biochemistry. 1989 May 16;28(10):4374-82. doi: 10.1021/bi00436a038.
9
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Biochim Biophys Acta. 1989 Jun 1;1008(1):102-7. doi: 10.1016/0167-4781(89)90175-9.
10
[Template-primer-dependent inactivation of DNA polymerase alpha from human placenta by 2',3'-epoxyadenosine-5'-triphosphate].[2',3'-环氧腺苷-5'-三磷酸对人胎盘DNA聚合酶α的模板引物依赖性失活作用]
Bioorg Khim. 1990 Feb;16(2):226-35.