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Comparison of interactions of 5'-derivatives of deoxyoctathymidylate with human DNA polymerize alpha and HIV reverse transcriptase.

作者信息

Khodyreva S N, Podust V N, Sergeev D S, Ivanova E M, Frolova E I, Koshkin A A, Godovikova T S, Zarytova V F, Ricchetti M, Lavrik O I

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk.

出版信息

Mol Biol Rep. 1993 Jun;18(1):43-7. doi: 10.1007/BF01006894.

DOI:10.1007/BF01006894
PMID:7694077
Abstract

Km and Vmax values for d(pT8) and its derivatives containing various 5'-end groups were estimated in the reaction of DNA polymerization alpha catalyzed by DNA polymerase alpha and HIV-RT. The effect of 5'-end modification of primer is more pronounced in the case of HIV-RT. Strong influence is observed for an intercalating (ethidium) group. The affinity of EtpT8 is 200-fold higher than that of d(pT8). Attachment of Phn-, Dnm- and Hem-groups results in the increase of affinity of modified primer from 10 up to 20 times. For DNA polymerase alpha the influence of modifiers on primer affinity is much weaker. The effect of 5'-end residues on the Vmax values is also more pronounced for HIV RT. The way to improve selective interaction of oligonucleotide derivatives with the primer site of HIV RT is suggested.

摘要

相似文献

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Comparison of interactions of 5'-derivatives of deoxyoctathymidylate with human DNA polymerize alpha and HIV reverse transcriptase.
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10
5'-derivatives of oligonucleotides as primers of DNA polymerization catalyzed by AMV reverse transcriptase and Klenow fragment of DNA polymerase 1.作为由禽成髓细胞瘤病毒逆转录酶和DNA聚合酶1的Klenow片段催化的DNA聚合反应引物的寡核苷酸5'-衍生物。
FEBS Lett. 1991 Apr 9;281(1-2):111-3. doi: 10.1016/0014-5793(91)80371-9.