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[沃洛德米尔·奥列克桑德罗维奇·别利采尔——一位天才科学家、杰出生物化学家,科学学派创始人]

[Volodymyr Oleksandrovych Bielitser--a gifted scientist and outstanding biochemist, a founder of the scientific school].

出版信息

Ukr Biokhim Zh (1999). 2006 May-Jun;78(3):5-135.

Abstract

Professor V. O. Belitser, Doctor of Science (biology), (30.09.1906, Ryazan, RF-04.03.1988 Kyiv, Ukraine), Member of the National Academy of Sciences of Ukraine, graduated from the physico-mathematical faculty of the Moscow University in speciality "physico-chemical biology". In 1934-1943 he worked at the All-Union Institute of Experimental Medicine (Moscow) where he was engaged in research of the relation between the respiratory system and glycolytic reactions in the animal tissues. V. O. Belitser established the effect of creatin on the muscular respiration on the role of creative phosphate in this process. He was the first to demonstrate that the anaerobic phosphorylation is bound to respiration. He investigated stechiometric relations between the joint phosphate binding and oxygen absorption and estimated thermodynamic importance of this process, he showed that the energy of electron transfer from the substrate to oxygen is a source of formation of three ATP molecules per one atom of absorbed oxygen. From 1944 to 1988 V. O. Belitser worked at the Institute of Biochemistry of the Academy of Sciences of the Ukr.SSR (Kyiv), where he headed the Laboratory of Enzymes (then proteins), and from 1966 he headed the Department of Protein Structure and Function; for a certain period (1969-1972) he headed the Institute as its director. Investigations of properties of native and denaturated proteins jointly with K. I. Kotkova led to the creation of blood substitute from blood serum proteins of cattle--BK-8. The school of V. O. Belitser is known by studying the molecular mechanism of one of the basic reactions of blood coagulation--fibrinogen transformation to fibrin, by finding out the organization and function of fibrinogen and fibrin. It was proved experimentally that the specific polymerization centres significance for the fibrin lattice formation are of essential significance for the fibrin lattice formation, that fibrinogen to fibrin transformation occurs in two stages--enzymatic and polymerizational ones. V. O. Belitser proposed the mechanism of fibrinogen transformation to fibrin, as soon as he had substantiated the kinetic theory of this reaction; domain structure of fibrinogen has been investigated. Such diagnostic tests as the methods of definition of the products of fibrinogen and fibrin splitting in urine (for differential diagnosis of cardiovascular diseases) were developed and put into medical practice under his guidance. V. O. Belitser and members of his school have published above 300 scientific works, prepared 5 doctors and 25 candidates of science. The selfless work of the scientists was honoured with high state awards--the Orders of Lenin, the Order of the Labour Red Banner, the Order of Oktober Revolution, that of Friendship of Peoples and with numerous medals.

摘要

V. O. 贝利采尔教授,生物学博士(1906年9月30日生于俄罗斯梁赞市,1988年3月4日卒于乌克兰基辅),乌克兰国家科学院院士,毕业于莫斯科大学物理数学系,专业为“物理化学生物学”。1934年至1943年,他在全苏实验医学研究所(莫斯科)工作,从事动物组织中呼吸系统与糖酵解反应之间关系的研究。V. O. 贝利采尔确定了肌酸对肌肉呼吸的影响以及磷酸肌酸在此过程中的作用。他首次证明无氧磷酸化与呼吸作用相关。他研究了联合磷酸结合与氧气吸收之间的化学计量关系,并评估了这一过程的热力学重要性,表明从底物到氧气的电子转移能量是每吸收一个氧原子形成三个ATP分子的来源。1944年至1988年,V. O. 贝利采尔在乌克兰苏维埃社会主义共和国科学院生物化学研究所(基辅)工作,他领导酶学实验室(后为蛋白质实验室),1966年起担任蛋白质结构与功能系主任;在一定时期(1969 - 1972年),他担任该研究所所长。他与K. I. 科特科娃共同对天然和变性蛋白质的特性进行研究,促成了用牛血清蛋白制造血液替代品——BK - 8。V. O. 贝利采尔学派以研究血液凝固的基本反应之一——纤维蛋白原转化为纤维蛋白的分子机制、揭示纤维蛋白原和纤维蛋白的结构与功能而闻名。实验证明,特定的聚合中心对纤维蛋白晶格形成具有至关重要的意义,纤维蛋白原向纤维蛋白的转化分两个阶段进行——酶促阶段和聚合阶段。V. O. 贝利采尔在证实该反应动力学理论后,提出了纤维蛋白原转化为纤维蛋白的机制;对纤维蛋白原的结构域结构进行了研究。在他的指导下,开发了诸如尿液中纤维蛋白原和纤维蛋白裂解产物测定方法等诊断测试(用于心血管疾病的鉴别诊断)并应用于医学实践。V. O. 贝利采尔及其学派成员发表了300多篇科学著作,培养了5名博士和25名副博士。科学家们的无私工作荣获了多项国家高级奖项——列宁勋章、劳动红旗勋章、十月革命勋章、友谊勋章以及众多奖章。

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