Auepemkiate Sauvarat, Thongsuksai Paramee, Boonyaphiphat Pleumjit
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat-Yai, Songkhla 90110, Thailand.
J Med Assoc Thai. 2006 Sep;89(9):1460-5.
Bowen's disease (BD) is a skin carcinoma in situ occurring over the entire body surface. It shares similar histopathological features with Bowenoid papulosis (BP) of the genitalia, but differs in etiology and clinical course. Increased p16(INK4A) (p16) tumor suppressor protein expression has been demonstrated in relation to the progression of cutaneous squamous neoplasms.
To evaluate the difference in p16 expression between Bowen's disease and Bowenoid papulosis.
Biopsies of 46 cases of BD in the period 1994 - 2003 and 14 cases of BP during 1987 - 2003 in the Anatomical Pathology Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Thailand were studied by immunohistochemical methods using the P16 kit (CINTec Histology Kit, clone E6H4, Code-Nr. K5334, DakoCytomation, Denmark). Nuclear/cytoplasmic immunoreactivity in more than 10% of neoplastic cells was considered positive.
P16 expression was positive in 37 of 46 BD cases (80.4%) which was higher than that of BP (6 of 14 cases or 42.9%) (p value < 0.05, Chi-square test). The expression among the three groups of BD: extragenital (28 of 35), chronic arsenical-related (7 of 8) and genital lesions (2 of 3) was not significantly different (p value = 0.734, Chi-square test).
P16 expression was more frequent in BD than BP. This suggests a possible association between p16 expression and tumorigenesis of these lesions.
鲍恩病（BD）是一种发生于全身体表的原位皮肤癌。它与生殖器鲍温样丘疹病（BP）具有相似的组织病理学特征，但在病因和临床病程方面有所不同。已证实p16（INK4A）（p16）肿瘤抑制蛋白表达的增加与皮肤鳞状肿瘤的进展有关。
评估鲍恩病和鲍温样丘疹病之间p16表达的差异。
对泰国宋卡王子大学医学院病理学系解剖病理科1994 - 2003年期间的46例BD活检标本以及1987 - 2003年期间的14例BP活检标本，使用P16试剂盒（CINTec组织学试剂盒，克隆E6H4，编号K5334，丹麦达科公司）通过免疫组织化学方法进行研究。肿瘤细胞中超过10%出现核/细胞质免疫反应性被视为阳性。
46例BD病例中有37例（80.4%）p16表达呈阳性，高于BP（14例中有6例，即42.9%）（p值<0.05，卡方检验）。BD的三组之间的表达：生殖器外（35例中的28例）、慢性砷相关（8例中的7例）和生殖器病变（3例中的2例）无显著差异（p值 = 0.734，卡方检验）。
BD中p16表达比BP更常见。这表明p16表达与这些病变的肿瘤发生之间可能存在关联。
