Mahdadi R, Kenani A, Pommery N, Pommery J, Hénichart J P, Lhermitte M
Laboratoire de Toxicologie, Faculté de Pharmacie de Lille II, France.
Cancer Chemother Pharmacol. 1991;28(1):22-6. doi: 10.1007/BF00684951.
A sensitive and rapid linear-gradient, ion-paired, reversed-phase high-performance liquid chromatography technique using fluorescence detection was developed to quantify bleomycin (BLM) metabolites in the plasma of patients undergoing BLM therapy and in rat hepatocytes that had previously been incubated with 5 x 10(-5) M BLM. We could detect about 70 ng/ml using this procedure. BLM metabolites were assayed in the supernatant fractions of precipitated human plasma and in pellets of rat hepatocytes. Metabolite concentrations were below the level of detection in human plasma samples. In hepatocyte pellets, metabolites such as deamido-BLM A2 and deamido-BLM B2 were detected, indicating that isolated rat hepatocytes in culture can metabolize BLM analogues to the corresponding deamido-BLMs. The high-performance liquid chromatography procedure developed during this work can be used to study the metabolism of BLM in cell culture systems.
开发了一种灵敏且快速的线性梯度、离子对、反相高效液相色谱技术，采用荧光检测，用于定量接受博来霉素（BLM）治疗患者血浆及先前用5×10⁻⁵ M博来霉素孵育的大鼠肝细胞中博来霉素的代谢产物。使用该方法我们能够检测到约70 ng/ml。在沉淀的人血浆上清液部分和大鼠肝细胞沉淀中测定博来霉素代谢产物。代谢产物浓度在人血浆样本的检测水平以下。在肝细胞沉淀中，检测到脱氨基博来霉素A2和脱氨基博来霉素B2等代谢产物，表明培养的分离大鼠肝细胞可将博来霉素类似物代谢为相应脱氨基博来霉素。在这项工作中开发的高效液相色谱方法可用于研究细胞培养系统中博来霉素的代谢。
