Klett R P, Chovan J P, Danse I H
J Chromatogr. 1984 Oct 12;310(2):361-71. doi: 10.1016/0378-4347(84)80101-2.
A rapid, linear gradient chromatographic technique for separating and quantifying copper(II)-chelated bleomycin congeners is described. This method is also capable of separating divalent from trivalent metal chelates; determining the purity of many chemically modified bleomycins; and assaying bleomycin hydrolase activity on complex mixtures. Quantification at 280 nm is sensitive to 50 pmol and is linear at least up to 10 nmol per injection.
描述了一种用于分离和定量铜（II）螯合博来霉素同系物的快速线性梯度色谱技术。该方法还能够分离二价和三价金属螯合物；测定许多化学修饰博来霉素的纯度；并检测复杂混合物中博来霉素水解酶的活性。在280nm处进行定量，灵敏度可达50pmol，每次进样至少在10nmol范围内呈线性。
