Phan Bao H, Jin Weiwei, Topp Christopher N, Zhong Cathy X, Jiang Jiming, Dawe R Kelly, Parrott Wayne A
Department of Crop and Soil Sciences, University of Georgia, 111 Riverbend Road, Athens, GA 30602, USA.
Transgenic Res. 2007 Jun;16(3):341-51. doi: 10.1007/s11248-006-9041-3. Epub 2006 Nov 14.
Rice was transformed with either long DNA-segments of random genomic DNA from rice, or centromere-specific DNA sequences from either maize or rice. Despite the repetitive nature of the transgenic DNA sequences, the centromere-specific sequences were inserted largely intact and behave as simple Mendelian units. Between 4 and 5% of bombarded callus clusters were transformed when bombarded with just pCAMBIA 1305.2. Frequency of recovery dropped to 2-3% when BACs with random genomic inserts were co-bombarded with pCAMBIA, and fell to less than 1% when BACs with centromeric DNA inserts and pCAMBIA were co-bombarded. A similar effect was noted on regeneration frequency. Differences in transformation ability, regeneration and behavior of plants transgenic for BACs with random genomic DNA inserts, as compared to those with centromeric DNA inserts, suggests functional differences between these two types of DNA.
用来自水稻的随机基因组DNA的长DNA片段，或来自玉米或水稻的着丝粒特异性DNA序列转化水稻。尽管转基因DNA序列具有重复性，但着丝粒特异性序列在很大程度上完整插入，并表现为简单的孟德尔单位。仅用pCAMBIA 1305.2轰击时，4%至5%的轰击愈伤组织团块被转化。当带有随机基因组插入片段的BAC与pCAMBIA共轰击时，转化频率降至2%至3%，当带有着丝粒DNA插入片段的BAC与pCAMBIA共轰击时，转化频率降至不到1%。在再生频率上也观察到了类似的效果。与带有着丝粒DNA插入片段的BAC相比，带有随机基因组DNA插入片段的BAC转基因植物在转化能力、再生和行为上的差异表明这两种类型的DNA之间存在功能差异。
