Shahrabadi M S, Yamamoto T
Can J Microbiol. 1975 Sep;21(9):1421-7. doi: 10.1139/m75-213.
Spherical dark inclusions were observed in both the cytoplasm and nuclei of infectious canine laryngotracheitis (ICL) adenovirus infected MDCK (Madin-Darby Canine Kidney) cells. The distribution of these inclusions in the cells appeared to indicate that they were formed in both the cytoplasm and in the nucleus at about the same time and there did not appear to be movement of these inclusions between the cytoplasm and the nucleus during the early stages of infection. Morphological appearance, 3H-leucine autoradiography, and immunoferritin labelling showed that the cytoplasmic inclusions were similar in nature to the dark nuclear inclusions, and contained the adenovirus hexon antigen, but not the penton base and fiber antigens.
在感染传染性犬喉气管炎(ICL)腺病毒的MDCK(麦迪逊-达比犬肾)细胞的细胞质和细胞核中均观察到球形深色包涵体。这些包涵体在细胞中的分布似乎表明它们在细胞质和细胞核中大约同时形成,并且在感染早期这些包涵体在细胞质和细胞核之间似乎没有移动。形态学外观、³H-亮氨酸放射自显影和免疫铁蛋白标记显示,细胞质包涵体在性质上与深色核包涵体相似,并且含有腺病毒六邻体抗原,但不含有五邻体基底和纤维抗原。