[Cytopathology of cell cultures infected with adenovirus and with a biologically active preparation of its DNA].

Comparative characteristics of cytopathic changes in the cell cultures infected with adenovirus and with a biologically active preparation of adenovirus DNA are presented. The pathological process in the cells infected with the infectious DNA differs from that after virus infection by a shortened time of DNA penetration into the cell and a shortened latent period of infection development. The development of the pathological process in the cell cultures was accompanied by a complex of cytological changes with predominant affection of the nuclei and formation of intranuclear inclusions staining green with acridine orange. The distinctive feature of these changes was the time of their appearance: in cultures infected with the virus inclusions were formed within 12-48 hours, in those infected with DNA within 9-18 hours. The fluorescent antibdoy procedure revealed a virus antigen in these cultures induced by DNA preparationand by the virus, at different stages of its accumulation. The nucleoplasm was the site of specific antigens synthesis. The distribution of the virus antigen in the infected cells corresponds to the morphological pictures of intranuclear inclusions at later stages of infection which confirms the viral nature of the latter. The dynamics of the development of cytological changes and intranuclear inclusions in the infected cells as well as synthesis of a specific antigen in them are directly related to the quantitative accumulation of the virus.