Parham D M, Webber B, Holt H, Williams W K, Maurer H
Department of Pathology, Jude Children's Research Hospital, Memphis, Tennessee.
Cancer. 1991 Jun 15;67(12):3072-80. doi: 10.1002/1097-0142(19910615)67:12<3072::aid-cncr2820671223>3.0.co;2-z.
The authors assessed a panel of immunohistochemical stains against 109 pediatric solid tumors, primarily rhabdomyosarcomas, under the auspices of the Intergroup Rhabdomyosarcoma Study. Fresh tumor tissue received from participating organizations was divided into portions that were either frozen or fixed in formalin, alcohol, or B5. Immunostaining was performed by the avidin-biotin complex method using monoclonal antibodies to desmin, neurofilaments, vimentin, cytokeratin, and leukocyte common antigen on cryostat sections. Tissue was also embedded in paraffin and stained with antimuscle-specific actin (MSA) and polyclonal antibodies to desmin, creatine kinase M subunit (CKM), myoglobin, and neuron-specific enolase (NSE). Antidesmin staining of cryostat sections was the most sensitive indicator of rhabdomyosarcoma (58 of 62 specimens positive). Results with this reagent in alcohol-fixed and formalin-fixed tissue were similar (46 of 56 positive versus 43 of 56 positive, respectively) and comparable with results with anti-MSA in formalin-fixed tissue (43 of 55 positive). However, the proportion of cells stained by antidesmin was higher in alcohol-fixed tissue than in formalin-fixed tissue. Staining with antimyoglobin and anti-CKM was much less satisfactory, with positivity rates of 17 of 37 and 11 of 57, respectively, in formalin-fixed rhabdomyosarcomas. Immunostaining of muscle markers revealed evidence of myogenesis in six undifferentiated sarcomas and in two sarcomas with inadequate histologic study on hematoxylin-eosin-stained sections. However, positivity was also noticed in samples of fibromatosis, Wilms' tumor, ectomesenchyoma, peripheral primitive neuroectodermal tumor, renal rhabdoid tumor, myositis ossificans, malignant fibrous histiocytoma, and embryonal sarcoma of the liver. The authors conclude that combined use of antidesmin and anti-MSA enhances the diagnosis of childhood sarcomas, especially when employed with other techniques such as electron microscopic study.
在横纹肌肉瘤协作组研究的支持下，作者对一组免疫组化染色方法进行了评估，该评估针对109例儿童实体瘤，主要是横纹肌肉瘤。从参与组织获得的新鲜肿瘤组织被分成若干部分，一部分冷冻保存，另一部分用福尔马林、酒精或B5固定。采用抗生物素蛋白-生物素复合物方法，使用抗结蛋白、神经丝、波形蛋白、细胞角蛋白和白细胞共同抗原的单克隆抗体，对冰冻切片进行免疫染色。组织也被包埋在石蜡中，并用抗肌肉特异性肌动蛋白（MSA）以及抗结蛋白、肌酸激酶M亚基（CKM）、肌红蛋白和神经元特异性烯醇化酶（NSE）的多克隆抗体进行染色。冰冻切片的抗结蛋白染色是横纹肌肉瘤最敏感的指标（62个标本中有58个阳性）。该试剂在酒精固定组织和福尔马林固定组织中的结果相似（分别为56个中有46个阳性和56个中有43个阳性），与福尔马林固定组织中抗MSA的结果相当（55个中有43个阳性）。然而，酒精固定组织中被抗结蛋白染色的细胞比例高于福尔马林固定组织。抗肌红蛋白和抗CKM染色的效果要差得多，在福尔马林固定的横纹肌肉瘤中，阳性率分别为37个中有17个和57个中有11个。肌肉标志物的免疫染色显示，在6例未分化肉瘤和2例苏木精-伊红染色切片组织学研究不充分的肉瘤中存在肌发生的证据。然而，在纤维瘤病、肾母细胞瘤、外胚层间叶瘤、外周原始神经外胚层肿瘤、肾横纹肌样瘤、骨化性肌炎、恶性纤维组织细胞瘤和肝胚胎性肉瘤的样本中也发现了阳性。作者得出结论，联合使用抗结蛋白和抗MSA可提高儿童肉瘤的诊断水平，尤其是与电子显微镜检查等其他技术联合使用时。
