Schmidt R A, Cone R, Haas J E, Gown A M
Department of Pathology, University Hospital, University of Washington, Seattle 98195.
Am J Pathol. 1988 Apr;131(1):19-28.
The authors have recently developed a monoclonal antibody, HHF35, that recognizes the muscle-specific isoforms of actin. To determine its potential usefulness in the differential diagnosis of "small, round, blue cell" tumors of childhood, they immunolabeled formalinor B-5-fixed tissue sections from known cases of rhabdomyosarcoma or rhabdomyoma (30), neuroblastoma (9), retinoblastoma (2), and Ewing's sarcoma (9) with HHF35 and with antibodies to creatine kinase M, myoglobin, vimentin, and neuron-specific enolase. HHF35 reacted with 29 of 30 cases of rhabdomyosarcoma, whereas antibodies to creatine kinase M and myoglobin were positive on only 12 and 7 tumors, respectively. HHF35 did not react with any case of neuroblastoma, retinoblastoma, or Ewing's sarcoma when the antibody diluent contained 50 mM EDTA. These results indicate that HHF35 is a highly sensitive and specific marker for myogenic differentiation and that it will be useful in the differential diagnosis of rhabdomyosarcomas.
作者最近研制出一种单克隆抗体HHF35，它能识别肌动蛋白的肌肉特异性同工型。为了确定其在儿童“小圆蓝细胞”肿瘤鉴别诊断中的潜在用途，他们用HHF35以及针对肌酸激酶M、肌红蛋白、波形蛋白和神经元特异性烯醇化酶的抗体，对横纹肌肉瘤或横纹肌瘤（30例）、神经母细胞瘤（9例）、视网膜母细胞瘤（2例）和尤因肉瘤（9例）的已知病例的福尔马林或B - 5固定组织切片进行免疫标记。HHF35与30例横纹肌肉瘤中的29例发生反应，而针对肌酸激酶M和肌红蛋白的抗体分别仅在12例和7例肿瘤上呈阳性。当抗体稀释液含有50 mM EDTA时，HHF35与任何神经母细胞瘤、视网膜母细胞瘤或尤因肉瘤病例均无反应。这些结果表明，HHF35是一种用于肌源性分化的高度敏感和特异的标志物，并且它将有助于横纹肌肉瘤的鉴别诊断。
