Petrescu Amelia, Mârzan Liliana, Codreanu O, Niculescu L
Pathology Department, "Prof. dr. Theodor Burghele" Hospital, Bucharest.
Rom J Morphol Embryol. 2006;47(2):143-6.
THE AIM of our study was to evaluate the prognostic significance of p53 protein immunoreactivity for prostate cancer and to determine whether p53 immunoreactivity correlates with the Gleason tumor grade in primary adenocarcinoma. Prostate fragments were fixed in 10% formalin, paraffin-embedded, sectioned and standard Hematoxylin-Eosin stained, then examined using histological grade (Gleason system). P53 expression was studied using immunohistochemistry with monoclonal antibody anti-p53, 1 : 100 (BIOX) on tissue samples obtained during transurethral electroresection, adenomectomy or needle biopsy in 30 patients with prostate carcinoma: group 1 (n = 7) Gleason score 5, group 2 (n = 10) Gleason score 6, group 3 (n = 11) Gleason score 7, group 4 (n = 2) Gleason score 8. Also, we noted the cases with high grade prostatic intraepithelial neoplasia (high grade PIN). All specimens prior to initiation of any treatment were submitted for this study. Staining was defined as positive for p53 whenever any specific nuclear staining was detected. We considered tumors to overexpress p53 protein only when strong nuclear staining was present. Cases exhibiting weak or equivocal nuclear staining were classified as negative, as were cases with extremely rare isolated positive nuclei. A semiquantitative scoring system was employed to assess the level of p53 reactivity. Six of 17 (35.2%) moderately differentiated tumors (Gleason score 5-6) and five of 13 (38.4%) moderate to poorly differentiated (Gleason score 7 and above) revealed strong nuclear positivity for p53. In addition, we noted occasional p53 reactivity in high-grade PIN.
We interpret these data to demonstrate a positive association between p53 reactivity and higher Gleason grade tumors; p53 might be an independent prognostic indicator among metastatic risk cases.
我们研究的目的是评估p53蛋白免疫反应性对前列腺癌的预后意义，并确定p53免疫反应性是否与原发性腺癌的Gleason肿瘤分级相关。前列腺组织碎片用10%福尔马林固定，石蜡包埋，切片并进行标准苏木精-伊红染色，然后使用组织学分级（Gleason系统）进行检查。采用抗p53单克隆抗体（1:100，BIOX）免疫组化法研究30例前列腺癌患者经尿道电切术、腺瘤切除术或穿刺活检获取的组织样本中p53的表达：第1组（n = 7）Gleason评分为5分，第2组（n = 10）Gleason评分为6分，第3组（n = 11）Gleason评分为7分，第4组（n = 2）Gleason评分为8分。此外，我们还记录了高级别前列腺上皮内瘤变（高级别PIN）的病例。所有在开始任何治疗前的标本都提交用于本研究。只要检测到任何特异性核染色，p53染色即定义为阳性。仅当存在强核染色时，我们才认为肿瘤过度表达p53蛋白。显示弱或不明确核染色的病例以及极少数孤立阳性核的病例均分类为阴性。采用半定量评分系统评估p53反应性水平。17例中度分化肿瘤（Gleason评分5 - 6分）中的6例（占35.2%）以及13例中至低分化肿瘤（Gleason评分7分及以上）中的5例（占38.4%）显示p53强核阳性。此外，我们在高级别PIN中偶尔也观察到p53反应性。
我们解释这些数据表明p53反应性与较高Gleason分级肿瘤之间存在正相关；p53可能是转移风险病例中的一个独立预后指标。
