Department of pathology. Internal security forces hospital, Tunis, Tunisia.
Department of urology. Internal security forces hospital, Tunis, Tunisia.
Tunis Med. 2024 Feb 5;102(2):111-115. doi: 10.62438/tunismed.v102i2.4730.
The p53 gene mutation is one of the most common genetic alterations in many cancers. In prostate cancer (PCa), it has been associated with a poor prognosis, tumor progression and aggressiveness. P53 mutation induces an abnormal protein expression in related tissues.
This study aimed to assess p53 expression using immunohistochemistry in PCa and to discuss its prognostic value.
We have retrospectively collected all cases of PCa diagnosed in our pathology department between 2012 and 2022. An automatized immunohistochemical analysis was performed using monoclonal p53 antibody. For each case, we assessed the proportion of positive cells and the intensity of staining. P53 expression was considered abnormal when it was totally negative or overexpressed (>=50% of positive cells).
Twenty-four cases have been selected. Abnormal p53 expression was found in 42% of cases (P53 was overexpressed in 6cases and totally negative in 4 cases). Mean age of patients with p53 abnormal expression was 70years old. Patients with p53 abnormal expression had Gleason score >7 in 5 cases, ISUP grade >2 in 3 cases, peri-neural invasion in 8cases, capsule invasion in 9cases. All patients with p53 overexpression developed androgen resistance (p<0.01).
An aberrant expression profile of the p53 protein was observed in 42% of cases, and a statistically significant association was found with androgen resistance. Our results suggest a potential prognostic role of p53 in PCa.
p53 基因突变是许多癌症中最常见的遗传改变之一。在前列腺癌（PCa）中，它与预后不良、肿瘤进展和侵袭性有关。p53 突变会导致相关组织中异常蛋白表达。
本研究旨在通过免疫组织化学方法评估 PCa 中 p53 的表达，并探讨其预后价值。
我们回顾性地收集了 2012 年至 2022 年间在我们病理科诊断的所有 PCa 病例。使用单克隆 p53 抗体进行自动免疫组织化学分析。对于每个病例，我们评估了阳性细胞的比例和染色强度。当 p53 表达完全阴性或过表达（>=50%的阳性细胞）时，认为其表达异常。
共选择了 24 例病例。异常 p53 表达在 42%的病例中发现（6 例病例 p53 过表达，4 例病例完全阴性）。p53 表达异常患者的平均年龄为 70 岁。p53 表达异常的患者中有 5 例 Gleason 评分>7，3 例 ISUP 分级>2，8 例有神经周围侵犯，9 例有包膜侵犯。所有 p53 过表达的患者均发生雄激素抵抗（p<0.01）。
在 42%的病例中观察到 p53 蛋白表达异常，与雄激素抵抗有统计学显著关联。我们的结果表明 p53 在 PCa 中可能具有潜在的预后作用。
