Taktashev R E, Suvorova A V, Miller V E, Kolesnikova O I
Lab Delo. 1990(12):59-61.
The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
所建议的测量血浆肝素的方法基于肝素增强抗凝血酶III（AT-III）抗凝血酶活性的能力，AT-III是主要的Xa因子和凝血酶抑制剂。该方法包括在有和没有硫酸鱼精蛋白的情况下测量血浆AT-III活性，硫酸鱼精蛋白会破坏肝素-AT-III复合物。以校准曲线上肝素-AT-III复合物活性与AT-III自身活性的差异来确定每毫升血浆中肝素的含量（单位）。该方法灵敏度足够高，能够记录宽范围内（血浆中从0.01单位/毫升至0.75单位/毫升）的肝素浓度。
