Nenci G G, Berrettini M, Agnelli G, Parise P
Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):519-25.
Titration assay of heparin with protamine sulfate permits the quantitative measurement of the concentration of the heparin molecule; on the contrary, the amidolytic method allows only a determination of the biological activity of heparin because it is subjected to the interferences of antiheparin factors. These may even be assayed with the amidolytic method by the evaluation of the residual activity after the addition to plasma of known amounts of heparin. The combined used of the two methods may prove useful in the assessment of concentration and specific activity of unknown heparin solutions in mediums free of antiheparin activities.
用硫酸鱼精蛋白对肝素进行滴定测定可定量测量肝素分子的浓度；相反，酰胺水解法只能测定肝素的生物活性，因为它会受到抗肝素因子的干扰。甚至可以通过在血浆中加入已知量的肝素后评估残余活性，用酰胺水解法来测定这些抗肝素因子。在评估不含抗肝素活性的介质中未知肝素溶液的浓度和比活性时，联合使用这两种方法可能会被证明是有用的。
