Khare S K, Vaidya S, Gupta M N
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas.
Appl Biochem Biotechnol. 1991 Mar;27(3):205-16. doi: 10.1007/BF02921536.
When a protein is aggregated by chemical crosslinking inside Sephadex beads of appropriate pore size, it gets trapped inside the beads. The above approach was used for immobilization of beta-galactosidase, acid phosphatase, trypsin, and concanavalin A. It was found to be a simple, convenient, and fast method for immobilization of proteins.
