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交联伴刀豆球蛋白A - O -(二乙氨基乙基)纤维素——一种用于伴刀豆球蛋白A相互作用糖蛋白的亲和介质。

Crosslinked concanavalin A-O-(diethylaminoethyl)-cellulose--an affinity medium for concanavalin A-interacting glycoproteins.

作者信息

Kamra A, Gupta M N

机构信息

Chemistry Department, Indian Institute of Technology, New Delhi.

出版信息

Anal Biochem. 1987 Aug 1;164(2):405-10. doi: 10.1016/0003-2697(87)90511-2.

Abstract

When concanavalin A (Con A) is reacted with a low concentration of glutaraldehyde, the product formed strongly binds to DEAE-cellulose. Thus, the resultant material can be used as an affinity medium for those glycoproteins which interact with Con A. This affinity medium is easy to prepare, has a capacity comparable to that of similar commercially available affinity media, and is stable for up to at least 6 months.

摘要

当伴刀豆球蛋白A(Con A)与低浓度的戊二醛反应时,形成的产物能与二乙氨基乙基纤维素(DEAE - 纤维素)强烈结合。因此,所得材料可作为与Con A相互作用的糖蛋白的亲和介质。这种亲和介质易于制备,其容量与类似的市售亲和介质相当,并且至少稳定6个月。

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