Guo H X, Cun W, Liu L D, Dong S Z, Wang L C, Dong C H, Li Q H
Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, 650118, China.
Cell Prolif. 2006 Dec;39(6):507-18. doi: 10.1111/j.1365-2184.2006.00408.x.
Herpes simplex virus (HSV)-1 stimulation-related gene 1 (HSRG1) protein expression is induced in HSV-1 infected cells. We found that HSRG1 interacts with SV40 large T antigen (LT) in yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC) assay. This interaction alters LT's regulation of the SV40 promoter and its ability to influence the cell cycle. Choramphenicol acetyl-transferase (CAT) assays revealed that initiation of gene transcription by LT is changed by HSRG1 expression. HSRG1 inhibits the ability of LT to activate SV40 late gene transcription. Further data indicate that the ability of LT protein to stimulate S-phase entry is also inhibited by the expression of HSRG1. The results of a colony-forming assay suggested that expression of HSRG1 in cells transfected by LT gene decreased the rate of colony formation. Yeast two-hybrid beta-galactosidase assay revealed that amino acid residues 132-450 in LT bind HSRG1.
单纯疱疹病毒1型(HSV-1)刺激相关基因1(HSRG1)蛋白表达在HSV-1感染的细胞中被诱导。我们发现在酵母双杂交试验和双分子荧光互补(BiFC)试验中,HSRG1与猿猴病毒40大T抗原(LT)相互作用。这种相互作用改变了LT对SV40启动子的调控及其影响细胞周期的能力。氯霉素乙酰转移酶(CAT)试验表明,HSRG1的表达改变了LT引发的基因转录。HSRG1抑制LT激活SV40晚期基因转录的能力。进一步的数据表明,HSRG1的表达也抑制了LT蛋白刺激细胞进入S期的能力。集落形成试验结果表明,在转染了LT基因的细胞中HSRG1的表达降低了集落形成率。酵母双杂交β-半乳糖苷酶试验表明,LT中的氨基酸残基132 - 450与HSRG1结合。