Okada Atsumasa, Tominaga Mitsutoshi, Horiuchi Makoto, Tomooka Yasuhiro
Department of Biological Science and Technology, and Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Biochem Biophys Res Commun. 2007 Jan 5;352(1):158-63. doi: 10.1016/j.bbrc.2006.10.176. Epub 2006 Nov 7.
Class 3 semaphorin acts as a guidance clue for both cell migration and nerve fiber projection. The signal of class 3 semaphorin travels via a receptor complex consisting of neuropilins and Plexin-A subfamily. Although it has been reported that class 3 semaphorin acts as a repellent for oligodendrocyte precursor cells (OPCs), which migrate actively during brain development, the expression of Plexin-A subfamily has not been reported in OPCs yet. Therefore, it is currently unclear how semaphorin signals can travel in OPCs. In the present study, the expression of Plexin-A4 (PlexA4) was first demonstrated in a newly established OPC line and OPCs in developing brain. In the OPC line, repulsion for process extension was caused by both Sema3A and Sema6A, and the effect of the semaphorins was diminished in cells expressing PlexA4 lacking the cytoplasmic domain. These results strongly suggest that PlexA4 expressed in OPCs acts as a mediator of semaphorin signals.
3类信号素作为细胞迁移和神经纤维投射的导向线索。3类信号素的信号通过由神经纤毛蛋白和丛状蛋白A亚家族组成的受体复合物传导。尽管有报道称3类信号素对少突胶质前体细胞（OPC）起排斥作用，少突胶质前体细胞在脑发育过程中会积极迁移，但丛状蛋白A亚家族在少突胶质前体细胞中的表达尚未见报道。因此，目前尚不清楚信号素信号如何在少突胶质前体细胞中传导。在本研究中，首次在新建立的少突胶质前体细胞系和发育中的脑中的少突胶质前体细胞中证实了丛状蛋白A4（PlexA4）的表达。在少突胶质前体细胞系中，Sema3A和Sema6A均引起突起延伸的排斥反应，并且在表达缺乏胞质结构域的PlexA4的细胞中，信号素的作用减弱。这些结果强烈表明，在少突胶质前体细胞中表达的PlexA4作为信号素信号的介质。
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