Piza Adriana S Toledo, Pereira Alessandra Rael, Terreran Maria Thereza, Mozzer Otto, Tanuri Amílcar, Brandão Paulo Eduardo, Richtzenhain Leonardo José
Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, CEP 05508-270, São Paulo, SP, Brazil.
Prev Vet Med. 2007 Mar 17;78(3-4):239-45. doi: 10.1016/j.prevetmed.2006.10.009. Epub 2006 Nov 15.
We used a p26 recombinant protein (p26r) from equine infectious-anemia virus (EIAV) expressed in Escherichia coli as antigen to standardize an agar-gel immunodiffusion (AGIDp26r) test and an indirect ELISA (ELISAp26r) for the detection of antibodies against EIAV in 720 equine sera from Brazil. We evaluated the tests's relative diagnostic sensitivities (relSe) and relative diagnostic specificities (relSp) against a commercial AGID kit (Idexx, USA). We used three sera panels: panel A--196 AGID-negative sera from an AIE non-endemic controlled area; panel B--194 AGID-negative sera from an AIE endemic area and panel C--330 AGID-positive sera from an AIE endemic area. ELISAp26r cut-off value was defined with TG-ROC using sera from panels A and C. AGIDp26r showed an agreement of 100% with the commercial kit. When applied to sera from panels A and C, ELISAp26r showed an agreement of 100% with the kit, but, although relSe was 100% for panel C, the ELISAp26r had relSp of 93.3%.
我们使用在大肠杆菌中表达的来自马传染性贫血病毒（EIAV）的p26重组蛋白（p26r）作为抗原，对用于检测来自巴西的720份马血清中抗EIAV抗体的琼脂凝胶免疫扩散试验（AGIDp26r）和间接ELISA（ELISAp26r）进行标准化。我们针对一种商业AGID试剂盒（美国Idexx公司）评估了这些试验的相对诊断敏感性（relSe）和相对诊断特异性（relSp）。我们使用了三个血清组：A组——来自一个AIE非流行控制区的196份AGID阴性血清；B组——来自一个AIE流行区的194份AGID阴性血清；C组——来自一个AIE流行区的330份AGID阳性血清。使用来自A组和C组的血清通过TG-ROC确定ELISAp26r的临界值。AGIDp26r与商业试剂盒的一致性为100%。当应用于A组和C组的血清时，ELISAp26r与试剂盒的一致性为100%，但是，尽管C组的relSe为100%，ELISAp26r的relSp为93.3%。
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