用于检测马传染性贫血病毒抗体对纯化P26病毒蛋白的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified P26 viral protein.
作者信息
Shen D T, Gorham J R, McGuire T C
出版信息
Am J Vet Res. 1984 Aug;45(8):1542-3.
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of equine infectious anemia (EIA) antibody in horse sera. Purified P26 viral protein was the antigen; alkaline phosphatase linked to rabbit anti-horse immunoglobulin G was the conjugate. The ELISA detected EIA antibodies in horse sera as early as 11 to 14 days after experimental inoculations. There was full agreement between the results of ELISA and the agar-gel immunodiffusion tests on EIA proficiency test sera. The ELISA readily detected EIA antibody in horse sera that had weak positive reactions on agar-gel immunodiffusion.
开发了一种间接酶联免疫吸附测定法（ELISA），用于检测马血清中的马传染性贫血（EIA）抗体。纯化的P26病毒蛋白作为抗原；与兔抗马免疫球蛋白G连接的碱性磷酸酶作为共轭物。该ELISA早在实验接种后11至14天就能检测出马血清中的EIA抗体。ELISA结果与EIA能力验证试验血清的琼脂凝胶免疫扩散试验结果完全一致。该ELISA能够轻松检测出在琼脂凝胶免疫扩散试验中呈弱阳性反应的马血清中的EIA抗体。
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