Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Brasil.
J Virol Methods. 2012 Mar;180(1-2):62-7. doi: 10.1016/j.jviromet.2011.12.012. Epub 2011 Dec 30.
Equine infectious anemia (EIA) is an important viral infection affecting horses worldwide. The course of infection is accompanied generally by three characteristic stages: acute, chronic and inapparent. There is no effective EIA vaccine or treatment, and the control of the disease is based currently on identification of EIAV inapparent carriers by laboratory tests. Recombinant envelope protein (rgp90) was expressed in Escherichia coli and evaluated via enzyme-linked immunosorbent assay (ELISA). There was an excellent agreement (95.42%) between the ELISA results using rgp90 and agar gel immunodiffusion test results. AGID is considered the "gold-standard" serologic test for equine infectious anemia (EIA). After 1160 serum samples were tested, the relative sensitivity and specificity of the ELISA were 96.1% and 96.4%, respectively. Moreover, analysis diagnostic accuracy of the ELISA was performed. The ELISA proved robust. Furthermore, good reproducibility was observed for the negative controls and, positive controls for all plates tested.
马传染性贫血（EIA）是一种影响全球马匹的重要病毒感染。感染过程通常伴有三个特征阶段：急性、慢性和不显性。目前，尚无有效的 EIA 疫苗或治疗方法，该疾病的控制主要基于实验室检测鉴定 EIAV 不显性携带者。重组包膜蛋白（rgp90）在大肠杆菌中表达，并通过酶联免疫吸附试验（ELISA）进行评估。使用 rgp90 的 ELISA 结果与琼脂凝胶免疫扩散试验结果之间具有极好的一致性（95.42%）。AGID 被认为是马传染性贫血（EIA）的“金标准”血清学检测方法。在对 1160 份血清样本进行检测后，ELISA 的相对敏感性和特异性分别为 96.1%和 96.4%。此外，还对 ELISA 的诊断准确性进行了分析。该 ELISA 结果可靠，且所有测试板的阴性和阳性对照的重复性均良好。
