Ghaedi Mehrorang, Parham Hooshang, Moghadam Majid, Mortazavi Kianosh, Soylak Mustafa
Chemistry Department, Yasouj University, Yasouj 75914-353, Iran.
Ann Chim. 2006 Nov-Dec;96(11-12):689-96. doi: 10.1002/adic.200690071.
A simple, selective and rapid method for solid phase extraction and spectrophotometric determination of thiocyanate using a manganese (III) tetrakis (p-sulfonatophenyl) porphyrin, [Mn (TPPS) OAC] bound to Amberlite IR-400 has been developed. The influence of pH, amount of solid phase, sample matrix, type and amount of eluting agent and flow rates i.e. variables affecting the efficiency of the extraction system were evaluated and conditions of the sample, eluting solution and active phase were optimized. The maximal capacity was found to be as 1.16 microg mL(-1) for 1200 mL. Thiocyanate ions can be eluted quantitatively with 8 mL 0.3 M ferric chloride. The enrichment factor was 150. The linear range of the determination is between 0.4-2.0 microg mL(-1) for preconcentration method with a limit of detection of 2.8 ng mL(-1). The method has been successfully applied for determination of trace amounts of thiocyanate in tap water, saliva sample and a synthetic mixture.
已开发出一种简单、选择性好且快速的方法，用于使用与Amberlite IR - 400结合的四（对磺基苯基）卟啉锰（III）[Mn(TPPS)OAC]对硫氰酸盐进行固相萃取和分光光度测定。评估了pH值、固相量、样品基质、洗脱剂类型和用量以及流速等影响萃取系统效率的变量，并优化了样品、洗脱溶液和活性相的条件。发现1200 mL的最大容量为1.16 μg mL⁻¹。硫氰酸根离子可用8 mL 0.3 M氯化铁定量洗脱。富集因子为150。对于预浓缩方法，测定的线性范围在0.4 - 2.0 μg mL⁻¹之间，检测限为2.8 ng mL⁻¹。该方法已成功应用于自来水、唾液样品和合成混合物中痕量硫氰酸盐的测定。
