Sweetnam P M, Sanon H R, White L A, Brass B J, Jaye M, Whittemore S R
Miami Project, University of Miami School of Medicine, FL 33136.
J Neurochem. 1991 Jul;57(1):237-49. doi: 10.1111/j.1471-4159.1991.tb02121.x.
When spinal cord cultures from embryonic day 12 rats were cultured at low density, both acidic and basic fibroblast growth factors significantly increased neuronal survival and neurite outgrowth in a dose-dependent manner. The effects of acidic fibroblast growth factor were independent of heparin, in contrast to its mitogenic effects on both NIH3T3 cells and cerebral cortical astrocytes. In high-density cultures, acidic fibroblast growth factor increased choline acetyltransferase activity by 57%, glutamic acid decarboxylase activity by 58%, and aspartate aminotransferase activity by 65%. Basic fibroblast growth factor increased choline acetyltransferase activity by 73% and glutamic acid decarboxylase activity by 200% but decreased aspartate aminotransferase activity by 40%. Growing these cultures in the presence of a mitotic inhibitor did not significantly alter the effect of acidic or basic fibroblast growth factor on these enzyme activities. These results demonstrate that acidic and basic fibroblast growth factors differentially affect neurotransmitter enzyme levels of multiple classes of neurons, rather than having effects on a single neuronal population.
当将来自胚胎第12天大鼠的脊髓培养物进行低密度培养时，酸性和碱性成纤维细胞生长因子均以剂量依赖的方式显著提高神经元存活率和神经突生长。与酸性成纤维细胞生长因子对NIH3T3细胞和大脑皮质星形胶质细胞的促有丝分裂作用相反，其对神经元存活和神经突生长的影响不依赖于肝素。在高密度培养中，酸性成纤维细胞生长因子使胆碱乙酰转移酶活性提高57%，谷氨酸脱羧酶活性提高58%，天冬氨酸转氨酶活性提高65%。碱性成纤维细胞生长因子使胆碱乙酰转移酶活性提高73%，谷氨酸脱羧酶活性提高200%，但使天冬氨酸转氨酶活性降低40%。在有丝分裂抑制剂存在的情况下培养这些细胞，不会显著改变酸性或碱性成纤维细胞生长因子对这些酶活性的影响。这些结果表明，酸性和碱性成纤维细胞生长因子对多类神经元的神经递质酶水平有不同影响，而不是仅对单一神经元群体起作用。
