Matekwor Ahulu Evelyn, Andoh Hanaka, Nonaka Masanori
Forestry Research Institute of Ghana, UPO Box 63, UST, Kumasi, Ghana.
Soil Science Laboratory, Faculty of Agriculture, Niigata University, 8050, Ikarashi-2, Niigata, 950-2181, Japan.
Mycorrhiza. 2007 Mar;17(2):93-101. doi: 10.1007/s00572-006-0080-7. Epub 2006 Nov 17.
The arbuscular mycorrhizal (AM) morphology of three host plant species inoculated with single and mixed fungal culture and the distribution of AM fungal species in roots of the hosts treated with a mixed culture of AM fungi were determined. The aim was to investigate the effect of host plants and AM fungi on AM morphology of coexisting plant species. Noncolonized rooted cuttings of Hedera rhombea (Miq) Bean, Rubus parvifolius L., and Rosa multiflora Thunb. were inoculated with five fungal species as single and mixed culture inocula. The fungal species used were Gigaspora rosea and Scutellospora erythropa, previously isolated from H. rhombea; Acaulospora longula and Glomus etunicatum from R. parvifolius; and Glomus claroideum from both plant species. A few hyphal and arbusculate coils were seen in the mixed culture-inoculated roots of R. parvifolius; all fungal treatments produced this Paris-type AM in H. rhombea and Arum-type AM in R. parvifolius, and R. multiflora indicates that AM morphology is strongly controlled by the identity of the host plants used in this study. AM fungal rDNA was extracted separately from roots of each replicate plant species inoculated with the mixed fungal culture, amplified, cloned, sequenced, and analyzed to determine the AM fungal species and their respective proportions in roots of each plant species. Glomus etunicatum and G. claroideum of the family Glomaceae generally occurred more frequently in R. parvifolius and R. multiflora, which form Arum-types, whereas S. erythropa, of the family Gigasporaceae, was the most frequently detected species in H. rhombea, which produced Paris-type AM. Although the genotype of the plant species used appears to determine the AM morphologies formed, there was preferential association between the hosts and AM fungal inoculants.
测定了接种单一和混合真菌培养物的三种寄主植物的丛枝菌根（AM）形态，以及用AM真菌混合培养物处理的寄主植物根中AM真菌物种的分布。目的是研究寄主植物和AM真菌对共存植物物种AM形态的影响。用五种真菌物种作为单一和混合培养接种物对接种了Hedera rhombea（Miq）Bean、Rubus parvifolius L.和Rosa multiflora Thunb.的未定殖生根插条进行接种。所使用的真菌物种有先前从H. rhombea分离出的Gigaspora rosea和Scutellospora erythropa；从R. parvifolius分离出的Acaulospora longula和Glomus etunicatum；以及从两种植物物种中分离出的Glomus claroideum。在接种混合培养物的R. parvifolius根中观察到一些菌丝和丛枝线圈；所有真菌处理在H. rhombea中产生这种巴黎型AM，在R. parvifolius中产生天南星型AM，而R. multiflora表明AM形态受本研究中使用的寄主植物身份强烈控制。从接种混合真菌培养物的每个重复植物物种的根中分别提取AM真菌rDNA，进行扩增、克隆、测序和分析，以确定每个植物物种根中的AM真菌物种及其各自比例。Glomaceae科的Glomus etunicatum和G. claroideum通常在形成天南星型的R. parvifolius和R. multiflora中出现得更频繁，而Gigasporaceae科的S. erythropa是在产生巴黎型AM的H. rhombea中最常检测到的物种。虽然所使用的植物物种基因型似乎决定了形成的AM形态，但寄主和AM真菌接种剂之间存在优先关联。
