Guimarães Sergio Botelho, Santos Jefferson Menezes Viana, Aragão Alan Arruda, de Sandes Kimura Osamu, Barbosa Paulo Hudson Uchoa, de Vasconcelos Paulo Roberto Leitão
Department of Surgery, and Head, Experimental Surgery Laboratory, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Nutrition. 2007 Jan;23(1):76-80. doi: 10.1016/j.nut.2006.09.005. Epub 2006 Nov 16.
We investigated the effect of alpha-lipoic acid (LA) in rats that were subjected to torsion/detorsion of the spermatic cord in a comparative controlled experiment.
Forty-eight male Wistar rats, randomized in two groups, received intraperitoneal injections of LA (LA group; aqueous solution at 36 mg/kg of body weight per day) or equal volume of saline (control group) 21, 9, and 1 h before torsion of the spermatic cord. Rats in each group were distributed in four subgroups, each comprising six animals. All surgical procedures were performed under inhaled ether anesthesia. Ischemia was induced by 720-degree torsion of the spermatic cord for 3 h. The right testis was assessed through longitudinal scrotal incision. After each surgical procedure, scrotal incisions were closed with 4-0 nylon monofilament. Ipsilateral testes and arterial blood samples were collected at the end of ischemia and 1, 3, and 6 h after detorsion. Thiobarbituric acid-reactive substances and reduced glutathione levels (micromoles per gram of wet tissue) were assayed in testis. Total antioxidant power was measured in blood plasma.
LA pretreatment promoted a significant decrease in testicular concentrations of thiobarbituric acid-reactive substances and simultaneously induced an increase in reduced glutathione concentrations at all time points studied. Plasma total antioxidant power levels increased significantly during reperfusion (T-1) in LA-treated rats compared with control rats.
LA administered before torsion of the spermatic cord showed significant protective effects against ischemia/reperfusion injury by decreasing lipid peroxidation and regulating testicular reduced glutathione and plasma total antioxidant power levels.
在一项对比对照实验中，我们研究了α-硫辛酸（LA）对精索扭转/去扭转大鼠的影响。
48只雄性Wistar大鼠随机分为两组，在精索扭转前21小时、9小时和1小时分别腹腔注射LA（LA组；每天36mg/kg体重的水溶液）或等体积的生理盐水（对照组）。每组大鼠再分为四个亚组，每组六只动物。所有手术操作均在吸入乙醚麻醉下进行。通过将精索扭转720度3小时诱导缺血。通过阴囊纵向切口评估右侧睾丸。每次手术后，用4-0尼龙单丝缝合阴囊切口。在缺血结束时以及去扭转后1小时、3小时和6小时收集同侧睾丸和动脉血样本。检测睾丸中硫代巴比妥酸反应性物质和还原型谷胱甘肽水平（每克湿组织微摩尔数）。测定血浆中的总抗氧化能力。
LA预处理使睾丸中硫代巴比妥酸反应性物质的浓度显著降低，同时在所有研究时间点均使还原型谷胱甘肽浓度升高。与对照组大鼠相比，LA处理的大鼠在再灌注期间（T-1）血浆总抗氧化能力水平显著升高。
在精索扭转前给予LA，通过降低脂质过氧化以及调节睾丸还原型谷胱甘肽和血浆总抗氧化能力水平，对缺血/再灌注损伤显示出显著的保护作用。
