Poppema S, Hepperle B
Department of Laboratory Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
Am J Pathol. 1991 Jun;138(6):1479-84.
Monoclonal antibodies reactive with variable regions of the human T-cell receptor (TCR) may be used to detect populations of T lymphocytes with restricted V gene usage. The authors have studied a series of 44 T-cell lymphomas with a panel of seven reagents reactive with four different TCR beta-chain variable region families. The authors found that, with this limited panel, restricted V gene usage of the neoplastic cells could be demonstrated in 29% of TCR-positive lymphomas. Whereas this is somewhat higher than expected, no preferential use of specific families could be demonstrated. An additional, unexpected finding was that most large cell T-cell lymphomas did not express TCR despite the presence of cytoplasmic CD3. The findings indicate that with a somewhat expanded panel it should be feasible to demonstrate restricted V gene usage as an indicator of clonality in a majority of T-cell lymphomas in a manner similar to the application of anti-immunoglobulin subclass antibodies in the diagnosis of clonal B cell proliferations.
与人T细胞受体（TCR）可变区反应的单克隆抗体可用于检测V基因使用受限的T淋巴细胞群体。作者使用一组七种与四个不同TCRβ链可变区家族反应的试剂，研究了一系列44例T细胞淋巴瘤。作者发现，使用这一有限的试剂组，在29%的TCR阳性淋巴瘤中可证明肿瘤细胞存在V基因使用受限。虽然这略高于预期，但未显示出对特定家族的优先使用。另一个意外发现是，尽管存在细胞质CD3，但大多数大细胞T细胞淋巴瘤不表达TCR。这些发现表明，通过稍微扩大试剂组，以类似于应用抗免疫球蛋白亚类抗体诊断克隆性B细胞增殖的方式，证明V基因使用受限作为大多数T细胞淋巴瘤克隆性的指标应该是可行的。
