人类Vβ基因亚家族分析
Analysis of a human V beta gene subfamily.
作者信息
Siu G, Strauss E C, Lai E, Hood L E
出版信息
J Exp Med. 1986 Nov 1;164(5):1600-14. doi: 10.1084/jem.164.5.1600.
Abstract
We have isolated and sequenced five germline V beta gene segments that are homologous to the V region of the YT35 cDNA encoding the beta chain of the T cell antigen receptor from the tumor MOLT-3. One of these gene segments is identical to the YT35 V segment, and therefore is the corresponding germline V beta gene segment encoding the YT35 cDNA. The other four V beta members exhibit 77-98% homology to the YT35 V gene segment. Two of these V beta gene segments are pseudogenes. Analyses of the coding region sequences reveal that, although the V beta segments are very diverse, they are mutating at a rate comparable to that observed in most eukaryotic genes. Analyses of the genomic clones show that the spacing distance between germline V beta gene segments ranges from 3 kb to greater than 30 kb, and the entire V beta 8 subfamily appears to be linked by a total of no more than 110 kb of DNA.
摘要
我们从肿瘤细胞系MOLT-3中分离并测序了五个种系Vβ基因片段，它们与编码T细胞抗原受体β链的YT35 cDNA的V区同源。其中一个基因片段与YT35 V片段相同，因此是编码YT35 cDNA的相应种系Vβ基因片段。其他四个Vβ成员与YT35 V基因片段具有77%-98%的同源性。其中两个Vβ基因片段是假基因。对编码区序列的分析表明，尽管Vβ片段非常多样，但它们的突变率与大多数真核基因中观察到的突变率相当。对基因组克隆的分析表明，种系Vβ基因片段之间的间隔距离从3 kb到大于30 kb不等，整个Vβ8亚家族似乎总共由不超过110 kb的DNA相连。
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