用于检测伯氏考克斯氏体的实时聚合酶链反应检测法的评估

Evaluation of a real-time PCR assay to detect Coxiella burnetii.

作者信息

Klee Silke R, Ellerbrok Heinz, Tyczka Judith, Franz Tatjana, Appel Bernd

机构信息

Robert Koch-Institut, Centre for Biological Safety, Nordufer 20, 13353 Berlin, Germany.

出版信息

Ann N Y Acad Sci. 2006 Oct;1078:563-5. doi: 10.1196/annals.1374.111.

DOI:10.1196/annals.1374.111

PMID:17114778

Abstract

We evaluated real-time PCR assays for the detection of C. burnetii which targets sequences that are present either in one (icd) or in several copies (transposase of IS1111a) on the chromosome. The assays are highly sensitive, with reproducible detection limits of approximately 10 copies per reaction, at least 100 times more sensitive than capture ELISA, when performed on infected placenta material and specific for C. burnetii. The numbers of IS1111 elements in the genomes of 75 C. burnetii isolates were quantified by real-time PCR and proved to be highly variable.

摘要

我们评估了用于检测伯纳特立克次体的实时聚合酶链反应（PCR）检测方法，该方法针对的是染色体上以单拷贝（icd）或多拷贝（IS1111a转座酶）形式存在的序列。这些检测方法高度灵敏，每个反应的可重复检测限约为10个拷贝，在对感染的胎盘材料进行检测时，其灵敏度至少比捕获酶联免疫吸附测定（ELISA）高100倍，且对伯纳特立克次体具有特异性。通过实时PCR对75株伯纳特立克次体分离株基因组中的IS1111元件数量进行了定量，结果证明其变化很大。