Chen Hua-Wei, Ching Wei-Mei
Naval Medical Research Center, Silver Spring, MD, United States.
J Microbiol Methods. 2014 Dec;107:176-81. doi: 10.1016/j.mimet.2014.07.039.
Q fever is an important worldwide zoonosis that is caused by infection with Coxiella burnetii. We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the presence of the transposase gene insertion element IS1111a of C. burnetii. The sensitivity of this LAMP assay is very similar to quantitative PCR (qPCR) method with a detection limit at 25 copies of the gene, the equivalent of about one C. burnetii organism. Several methods for the detection of LAMP product were also performed to show the diverse way of detection which may be used in different settings depending on the user's infrastructure and resource.
Q热是一种重要的全球性人畜共患病，由贝氏柯克斯体感染引起。我们开发了一种环介导等温扩增（LAMP）检测方法，用于检测贝氏柯克斯体转座酶基因插入元件IS1111a的存在。这种LAMP检测方法的灵敏度与定量PCR（qPCR）方法非常相似，检测限为该基因的25个拷贝，相当于约一个贝氏柯克斯体生物体。还采用了几种检测LAMP产物的方法，以展示不同的检测方式，可根据用户的基础设施和资源在不同环境中使用。
