Risk J M, Flanagan B F, Johnson P M
Department of Immunology, University of Liverpool, England.
Hum Immunol. 1991 Mar;30(3):162-7. doi: 10.1016/0198-8859(91)90030-d.
Restriction fragment length polymorphism analysis of the human CD46 gene, which encodes a cell surface complement regulatory protein, has been performed using a 1.5-kb cDNA probe containing a complete CD46 coding sequence. The sum of the invariant band sizes indicates that this gene encompasses at least 35-kb genomic DNA. Polymorphisms were detected in normal individuals with both HindIII and EcoRI. Several of the polymorphic bands occurred significantly less frequently in recurrent spontaneous abortion individuals. Northern blot analysis has shown the predominant RNA transcript size to be 4.2 kb in trophoblast-derived cell lines and peripheral blood mononuclear cells, indicating these transcripts may contain an unusually long 3'-untranslated region.
利用包含完整CD46编码序列的1.5kb cDNA探针，对编码细胞表面补体调节蛋白的人类CD46基因进行了限制性片段长度多态性分析。不变带大小的总和表明该基因包含至少35kb的基因组DNA。在正常个体中用HindIII和EcoRI均检测到多态性。几个多态性条带在复发性自然流产个体中出现的频率明显较低。Northern印迹分析表明，在滋养层来源的细胞系和外周血单核细胞中，主要的RNA转录本大小为4.2kb，表明这些转录本可能含有异常长的3'非翻译区。
