Fujita M, Furukawa F, Horiguchi Y, Ueda M, Kashihara-Sawami M, Imamura S
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
J Invest Dermatol. 1991 Jul;97(1):65-72. doi: 10.1111/1523-1747.ep12478115.
The regional development of Langerhans cells (LC) and the formation of Birbeck granules (BG) were examined in human embryonic and fetal skin. Samples were obtained from multiple anatomic sites and stained with anti-CD36, anti-CD1a, and anti-HLA-DR antibody as well as Lag antibody specifically reactive to BG and some vacuoles of human LC. In the first trimester, CD36+ dendritic epidermal cells were identified before the appearance of CD1a+ cells and Lag+ cells. Some of the former co-expressed HLA-DR antigens but not CD1a antigens. In the second trimester, regional variations in LC development were observed. Epidermal LC of palms and soles reached a peak in number in the first trimester but were rarely detected after 18 weeks estimated gestation age (EGA), whereas, in other regions, their number increased with age. In the second trimester, CD1a+ cells and Lag+ cells were also identified in the epidermis, although Lag+ cells appeared later than CD1a+ cells. The Lag+ cells until 17 weeks EGA showed a variety of staining intensities and immunoelectron microscopy revealed that they contained various amounts of Lag-reactive BG. Flow cytometric analysis showed that relative amounts of Lag antigens in LC increased during the second trimester and that fetal LC of 18 weeks EGA expressed the same amounts of HLA-DR, CD1a, and Lag antigens as did adult human LC. In the dermis, in the second trimester, numerous CD36+ cells and HLA-DR+ cells were found, whereas CD1a+ cells and Lag+ cells were rarely detected. Taken together, it is suggested that HLA-DR+ dendritic cells acquire CD1a+ antigens first and then form BG after migration to the epidermis and that fetal LC are phenotypically mature in the second trimester.
研究了人胚胎和胎儿皮肤中朗格汉斯细胞（LC）的区域发育及伯贝克颗粒（BG）的形成。从多个解剖部位获取样本，并用抗CD36、抗CD1a、抗HLA - DR抗体以及对人LC的BG和一些液泡具有特异性反应的Lag抗体进行染色。在妊娠早期，CD36⁺树突状表皮细胞在CD1a⁺细胞和Lag⁺细胞出现之前就已被识别。前者中的一些共表达HLA - DR抗原，但不表达CD1a抗原。在妊娠中期，观察到LC发育存在区域差异。手掌和足底的表皮LC数量在妊娠早期达到峰值，但在估计妊娠年龄（EGA）18周后很少检测到，而在其他区域，其数量随年龄增加。在妊娠中期，表皮中也识别出了CD1a⁺细胞和Lag⁺细胞，尽管Lag⁺细胞比CD1a⁺细胞出现得晚。直到EGA 17周的Lag⁺细胞显示出多种染色强度，免疫电子显微镜显示它们含有不同数量的Lag反应性BG。流式细胞术分析表明，妊娠中期LC中Lag抗原的相对含量增加，EGA 18周的胎儿LC表达的HLA - DR、CD1a和Lag抗原量与成人LC相同。在真皮中，妊娠中期发现了大量CD36⁺细胞和HLA - DR⁺细胞，而很少检测到CD1a⁺细胞和Lag⁺细胞。综上所述，提示HLA - DR⁺树突状细胞首先获得CD1a⁺抗原，然后迁移到表皮后形成BG，并且胎儿LC在妊娠中期表型成熟。
