Ozon R, Bellé R, Serres C, Fouchet C
Mol Cell Endocrinol. 1975 Sep;3(3):221-31. doi: 10.1016/0303-7207(75)90046-5.
The ability of various steroids to induce maturation of Pleurodeles waltlii oocytes after incubation has been studied. Progesterone is metabolized during the course of maturation. All metabolites isolated are less efficient than progesterone for inducing germinal vesicle breakdown. Progesterone binding to the 'melanosome' fraction has been studied (KD = 4.5 X 10(-8) M at 4 degrees C). 20BETA-Hydroxy-pregn-4-en-3-one, the main progesterone metabolite isolated from the oocyte, also binds to the melanosome fraction, but with a lower affinity (KD = 1.6 X 10(-7) M at 4 degrees C). At high concentration 20beta-hydroxy-pregn-4-en-3-one induces maturation, but at low concentration it is a competitive inhibitor of progesterone. Progesterone metabolism in Pleurodeles oocytes can be interpreted as an inactivation process, and also as a mechanism for inhibitime progesterone action.
研究了多种甾体在孵育后诱导疣螈卵母细胞成熟的能力。孕酮在成熟过程中会发生代谢。分离出的所有代谢产物在诱导生发泡破裂方面的效率均低于孕酮。已对孕酮与“黑素小体”部分的结合进行了研究（4℃时KD = 4.5×10⁻⁸ M）。从卵母细胞中分离出的主要孕酮代谢产物20β-羟基孕-4-烯-3-酮也与黑素小体部分结合，但亲和力较低（4℃时KD = 1.6×10⁻⁷ M）。高浓度时，20β-羟基孕-4-烯-3-酮可诱导成熟，但低浓度时它是孕酮的竞争性抑制剂。疣螈卵母细胞中的孕酮代谢可被解释为一种失活过程，也可作为抑制孕酮作用的一种机制。
