Militello V, Vitrano E, Cupane A
Istituto di Fisica dell'Università, Palermo, Italy.
Biophys Chem. 1991 Feb;39(2):161-9. doi: 10.1016/0301-4622(91)85018-l.
We have studied the effects of organic cosolvents (monohydric alcohols and formamide) on the oxygen affinity of human fetal hemoglobin stripped of phosphates and have compared them with the effects of the same cosolvents on the oxygen affinity of human adult hemoglobin under the same experimental conditions. Our results confirm that, in fetal hemoglobin, the T in equilibrium R conformational equilibrium is more displaced toward the T conformation than in the adult form and indicate that increased electrostatic and hydrophobic protein-solvent interactions contribute to this effect. The data reported are discussed in terms of the known amino acid substitutions between the beta- and gamma-chains and an attempt is made to rationalize the results with a molecular mechanism based on the crystallographic structure of fetal deoxyhemoglobin.
我们研究了有机助溶剂（一元醇和甲酰胺）对去除磷酸盐的人胎儿血红蛋白氧亲和力的影响，并在相同实验条件下将其与相同助溶剂对人成人血红蛋白氧亲和力的影响进行了比较。我们的结果证实，在胎儿血红蛋白中，平衡R构象平衡中的T比成人形式更偏向T构象，表明增加的静电和疏水蛋白质-溶剂相互作用促成了这种效应。根据β链和γ链之间已知的氨基酸取代对所报道的数据进行了讨论，并试图用基于胎儿脱氧血红蛋白晶体结构的分子机制来解释这些结果。
