Cordone L, Cupane A, Vitrano E
J Mol Biol. 1986 May 20;189(2):353-9. doi: 10.1016/0022-2836(86)90515-2.
We have studied the effects of monohydric alcohols and formamide on the oxygen equilibrium of native and N-ethylmaleimide-treated human haemoglobin. Comparison of the results obtained for the two haemoglobins gives further and compelling evidence in favour of the model, proposed recently by our group, on the role played by the solvent in the conformational equilibria of haemoglobin; moreover the results provide direct functional evidence of the relevance of the electrostatic free energy of salt bridges to the T in equilibrium with R equilibrium of haemoglobin.
我们研究了一元醇和甲酰胺对天然及N - 乙基马来酰亚胺处理的人血红蛋白氧平衡的影响。对两种血红蛋白所得结果的比较，为我们小组最近提出的关于溶剂在血红蛋白构象平衡中所起作用的模型提供了进一步且令人信服的证据；此外，这些结果为盐桥静电自由能与血红蛋白T态和R态平衡相关性提供了直接的功能证据。
